EP 1240513 A2 20020918 - HIGH THROUGHPUT SCREENING (HTS) ASSAYS FOR PROTEIN VARIANTS WITH REDUCED ANTIBODY BINDING CAPACITY
Title (en)
HIGH THROUGHPUT SCREENING (HTS) ASSAYS FOR PROTEIN VARIANTS WITH REDUCED ANTIBODY BINDING CAPACITY
Title (de)
VERFAHREN MIT EINER HOHEN UMSATZRATE (HTS) MIT PROTEIN VARIANTEN REDUZIERTEM BINDUNGSKAPAZITÄT DER ANTIKÖRPEN
Title (fr)
ESSAI PAR CRIBLAGE A HAUT DEBIT
Publication
Application
Priority
- DK 0000682 W 20001208
- DK PA199901765 A 19991209
Abstract (en)
[origin: WO0131989A2] The present invention is to provide a method to perform assays that efficiently and accurately can screen large numbers of cell populations producing variants of a molecule of interest. The present invention relates more specifically to a method for screening a library of protein variants for functional variants with reduced antibody binding capacity, comprising the steps of: (i) generating a diversified library of protein variants starting from a relevant protein backbone, (ii) transforming the library into suitable host cells, (iii) culturing host cells, (iv) sampling each cell culture, (v) analysing a sample by determining the antibody binding capacity of the variant protein, (vi) analysing a sample by determining the functionality of the variant protein.
IPC 1-7
IPC 8 full level
C40B 30/04 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
C40B 30/04 (2013.01); G01N 33/6803 (2013.01); G01N 33/6842 (2013.01); G01N 33/6845 (2013.01)
Citation (search report)
See references of WO 0131989A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0131989 A2 20010510; WO 0131989 A3 20011115; AU 1852201 A 20010514; AU 779195 B2 20050113; CA 2394377 A1 20010510; EP 1240513 A2 20020918
DOCDB simple family (application)
DK 0000682 W 20001208; AU 1852201 A 20001208; CA 2394377 A 20001208; EP 00981185 A 20001208