Global Patent Index - EP 1240513 A2

EP 1240513 A2 2002-09-18 - HIGH THROUGHPUT SCREENING (HTS) ASSAYS FOR PROTEIN VARIANTS WITH REDUCED ANTIBODY BINDING CAPACITY

Title (en)

HIGH THROUGHPUT SCREENING (HTS) ASSAYS FOR PROTEIN VARIANTS WITH REDUCED ANTIBODY BINDING CAPACITY

Title (de)

VERFAHREN MIT EINER HOHEN UMSATZRATE (HTS) MIT PROTEIN VARIANTEN REDUZIERTEM BINDUNGSKAPAZITÄT DER ANTIKÖRPEN

Title (fr)

ESSAI PAR CRIBLAGE A HAUT DEBIT

Publication

EP 1240513 A2 (EN)

Application

EP 00981185 A

Priority

  • DK 0000682 W
  • DK PA199901765 A

Abstract (en)

[origin: WO0131989A2] The present invention is to provide a method to perform assays that efficiently and accurately can screen large numbers of cell populations producing variants of a molecule of interest. The present invention relates more specifically to a method for screening a library of protein variants for functional variants with reduced antibody binding capacity, comprising the steps of: (i) generating a diversified library of protein variants starting from a relevant protein backbone, (ii) transforming the library into suitable host cells, (iii) culturing host cells, (iv) sampling each cell culture, (v) analysing a sample by determining the antibody binding capacity of the variant protein, (vi) analysing a sample by determining the functionality of the variant protein.

IPC 1-7 (main, further and additional classification)

G01N 33/50; C07K 16/00; C12N 15/00; G01N 33/68

IPC 8 full level (invention and additional information)

C40B 30/04 (2006.01); G01N 33/68 (2006.01)

CPC (invention and additional information)

C40B 30/04 (2013.01); G01N 33/6803 (2013.01); G01N 33/6842 (2013.01); G01N 33/6845 (2013.01)

Citation (search report)

See references of WO 0131989A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

WO 0131989 A2 20010510; WO 0131989 A3 20011115; AU 1852201 A 20010514; AU 779195 B2 20050113; CA 2394377 A1 20010510; EP 1240513 A2 20020918

INPADOC legal status


2009-04-22 [18R] REFUSED

- Effective date: 20080715

2005-06-15 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20050429

2002-09-18 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20020709

2002-09-18 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

2002-09-18 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI