Global Patent Index - EP 1242619 A2

EP 1242619 A2 2002-09-25 - ASSAY FOR DETECTION OF HUMAN CFTR ALLELE VARIANTS USING SPECIFIC DIAGNOSTIC PRIMERS

Title (en)

ASSAY FOR DETECTION OF HUMAN CFTR ALLELE VARIANTS USING SPECIFIC DIAGNOSTIC PRIMERS

Title (de)

VERFAHREN ZUR DETEKTION HUMANER CFTR ALLELVARIANTEN MITTELS SPEZIFISCHER DIAGNOSTISCHER PRIMER

Title (fr)

METHODE DE DETECTION DES VARIANTS ALLELIQUES DU CFTR HUMAIN UTILISANT DES AMORCES DIGNOSTIQUES SPECIFIQUES

Publication

EP 1242619 A2 (EN)

Application

EP 00960854 A

Priority

  • GB 0003597 W
  • GB 9922527 A

Abstract (en)

[origin: WO0121833A2] A diagnostic method for the detection of the 5T, 7T and 9T alleles in intron (8) of the human CFTR gene which method comprises contacting a test sample of nucleic acid from an individual with a multiplex of diagnostic primers comprising (i) 5T variant primer 5'(N)nAAAGAC3', (ii) 7T variant primer 5'(N*)n*(N)nAAAAGC3' and (iii) 9T variant primer 5'(N*)n*(N)nAAAATC3', wherein N represents additional nucleotides which base pair with the corresponding genomic sequence in the respective allele and n is an integer between 10 and 30 and N* represents additional non-homologous nucleotides which do not base pair with the corresponding genomic sequence in the respective allele and n* is an integer between 5 and 60, in the presence of appropriate nucleotide triphosphates and an agent for polymerisation, such that a diagnostic primer is extended only when the corresponding allelic variant is present in the sample; and detecting the presence or absence of the allelic variant by reference to the presence or absence of a diagnostic primer extension product.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6883 (2013.01); C12Q 2600/172 (2013.01)

Citation (search report)

See references of WO 0121833A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 0121833 A2 20010329; WO 0121833 A3 20020725; AU 7302200 A 20010424; CA 2385920 A1 20010329; EP 1242619 A2 20020925; GB 9922527 D0 19991124

INPADOC legal status


2005-10-12 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20050401

2002-09-25 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20020411

2002-09-25 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2002-09-25 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI