EP 1242621 A2 20020925 - PRIMERS, IN PARTICULAR, FOR PRIMER-DEPENDENT NUCLEIC ACID SYNTHESIS PROCESSES AND NUCLEIC ACID AMPLIFICATION METHODS
Title (en)
PRIMERS, IN PARTICULAR, FOR PRIMER-DEPENDENT NUCLEIC ACID SYNTHESIS PROCESSES AND NUCLEIC ACID AMPLIFICATION METHODS
Title (de)
PRIMER, INSBESONDERE FÜR PRIMER-ABHÄNGIGE NUKLEINSÄURE-SYNTHESEPROZESSE UND NUKLEINSÄURE-AMPLIFIKATIONSVERFAHREN
Title (fr)
AMORCE, NOTAMMENT POUR PROCESSUS DE SYNTHESE D'ACIDE NUCLEIQUE ET AMPLIFICATIONS D'ACIDE NUCLEIQUE
Publication
Application
Priority
- DE 19963857 A 19991230
- EP 0013288 W 20001227
Abstract (en)
[origin: WO0149880A2] The invention relates to primers which exhibit a high specificity and efficiency during hybridization with a target nucleic acid. To this end, the invention provides that a high proportion of bases, which can form two hydrogen bridge-type bonds with the corresponding bases in a target molecule, are predominant on the 3'-end of the hybridizing part of the primer. The 5'-end of the hybridizing part advantageously comprises a high proportion of bases which can form three hydrogen bridge-type bonds with the corresponding bases in the target molecule. The inventive primers are suited for use in primer-dependent nucleic acid synthesis processes, such as DNA syntheses and nucleic acid amplification methods, in particular, isothermal amplification methods. The inventive primers make it possible to significantly increase the yield of a specific amplification product and to reduce the production of undesired byproducts.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6865 (2018.01)
CPC (source: EP)
C12Q 1/6865 (2013.01)
Citation (search report)
See references of WO 0149880A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0149880 A2 20010712; WO 0149880 A3 20011206; DE 19963857 A1 20010726; EP 1242621 A2 20020925
DOCDB simple family (application)
EP 0013288 W 20001227; DE 19963857 A 19991230; EP 00985259 A 20001227