Global Patent Index - EP 1242621 A2

EP 1242621 A2 2002-09-25 - PRIMERS, IN PARTICULAR, FOR PRIMER-DEPENDENT NUCLEIC ACID SYNTHESIS PROCESSES AND NUCLEIC ACID AMPLIFICATION METHODS

Title (en)

PRIMERS, IN PARTICULAR, FOR PRIMER-DEPENDENT NUCLEIC ACID SYNTHESIS PROCESSES AND NUCLEIC ACID AMPLIFICATION METHODS

Title (de)

PRIMER, INSBESONDERE FÜR PRIMER-ABHÄNGIGE NUKLEINSÄURE-SYNTHESEPROZESSE UND NUKLEINSÄURE-AMPLIFIKATIONSVERFAHREN

Title (fr)

AMORCE, NOTAMMENT POUR PROCESSUS DE SYNTHESE D'ACIDE NUCLEIQUE ET AMPLIFICATIONS D'ACIDE NUCLEIQUE

Publication

EP 1242621 A2 (DE)

Application

EP 00985259 A

Priority

  • DE 19963857 A
  • EP 0013288 W

Abstract (en)

[origin: WO0149880A2] The invention relates to primers which exhibit a high specificity and efficiency during hybridization with a target nucleic acid. To this end, the invention provides that a high proportion of bases, which can form two hydrogen bridge-type bonds with the corresponding bases in a target molecule, are predominant on the 3'-end of the hybridizing part of the primer. The 5'-end of the hybridizing part advantageously comprises a high proportion of bases which can form three hydrogen bridge-type bonds with the corresponding bases in the target molecule. The inventive primers are suited for use in primer-dependent nucleic acid synthesis processes, such as DNA syntheses and nucleic acid amplification methods, in particular, isothermal amplification methods. The inventive primers make it possible to significantly increase the yield of a specific amplification product and to reduce the production of undesired byproducts.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6865 (2013.01)

Combination set (CPC)

C12Q 1/6865 + C12Q 2525/185

Citation (search report)

See references of WO 0149880A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

WO 0149880 A2 20010712; WO 0149880 A3 20011206; DE 19963857 A1 20010726; EP 1242621 A2 20020925

INPADOC legal status


2004-01-02 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20030701

2002-09-25 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20020605

2002-09-25 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR