Global Patent Index - EP 1242804 A2

EP 1242804 A2 2002-09-25 - HIGH VISCOSITY SHEATH REAGENT FOR FLOW CYTOMETRY

Title (en)

HIGH VISCOSITY SHEATH REAGENT FOR FLOW CYTOMETRY

Title (de)

HOCHVISKOSES HÜLLENREAGENS FÜR DURCHFLUSS-ZYTOMETRIE

Title (fr)

REACTIF D'ENVELOPPE A VISCOSITE ELEVEE POUR CYTOMETRIE DE FLUX

Publication

EP 1242804 A2 (EN)

Application

EP 00989570 A

Priority

  • US 0035543 W
  • US 17357299 P

Abstract (en)

[origin: WO0148455A2] A flow cytometer for the analysis of large particles or small multicellular organisms may experience unstable flow because of the large diameter of the flow chamber. The use of a sheath fluid or a sheath and sample with addition of a viscosity-increasing agent to give a viscosity higher than that of water ensures that flow in the pre-analysis section will be fully developed laminar flow, and that the flow in the analysis section will be laminar. This allows accurate analysis and sorting of large particles or analysis and sorting of smaller particles at an increased rate of speed. Water-soluble polymers are preferred because they increase fluid velocity with negligible osmotic effects. A 0.9 weight % solution of polyvinyl pyrollidone with an average molecular weight of 1.3 million is particularly effective. Use of viscosity-increasing agents that cause minimal increases in surface tension of the fluid is also preferred.

IPC 1-7 (main, further and additional classification)

G01N 15/14

IPC 8 full level (invention and additional information)

G01N 15/14 (2006.01); G01N 15/00 (2006.01)

CPC (invention and additional information)

G01N 15/1404 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/1411 (2013.01)

Citation (search report)

See references of WO 0148455A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

WO 0148455 A2 20010705; WO 0148455 A3 20020510; AU 2606301 A 20010709; CA 2396015 A1 20010705; EP 1242804 A2 20020925; JP 2004500562 A 20040108

INPADOC legal status


2007-10-24 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20070502

2007-01-24 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20061221

2003-04-16 [RIN1] INVENTOR (CORRECTION)

- Inventor name: MOORE, RICHARD, CHANNING

2003-04-16 [RIN1] INVENTOR (CORRECTION)

- Inventor name: FERRANTE, ANTHONY

2002-09-25 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20020705

2002-09-25 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

2002-09-25 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL;LT;LV;MK;RO;SI