EP 1242804 A2 20020925 - HIGH VISCOSITY SHEATH REAGENT FOR FLOW CYTOMETRY
Title (en)
HIGH VISCOSITY SHEATH REAGENT FOR FLOW CYTOMETRY
Title (de)
HOCHVISKOSES HÜLLENREAGENS FÜR DURCHFLUSS-ZYTOMETRIE
Title (fr)
REACTIF D'ENVELOPPE A VISCOSITE ELEVEE POUR CYTOMETRIE DE FLUX
Publication
Application
Priority
- US 0035543 W 20001229
- US 17357299 P 19991229
Abstract (en)
[origin: WO0148455A2] A flow cytometer for the analysis of large particles or small multicellular organisms may experience unstable flow because of the large diameter of the flow chamber. The use of a sheath fluid or a sheath and sample with addition of a viscosity-increasing agent to give a viscosity higher than that of water ensures that flow in the pre-analysis section will be fully developed laminar flow, and that the flow in the analysis section will be laminar. This allows accurate analysis and sorting of large particles or analysis and sorting of smaller particles at an increased rate of speed. Water-soluble polymers are preferred because they increase fluid velocity with negligible osmotic effects. A 0.9 weight % solution of polyvinyl pyrollidone with an average molecular weight of 1.3 million is particularly effective. Use of viscosity-increasing agents that cause minimal increases in surface tension of the fluid is also preferred.
IPC 1-7
IPC 8 full level
G01N 15/14 (2006.01); G01N 15/00 (2006.01)
CPC (source: EP)
G01N 15/1404 (2013.01); G01N 15/01 (2024.01); G01N 2015/1411 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0148455 A2 20010705; WO 0148455 A3 20020510; AU 2606301 A 20010709; CA 2396015 A1 20010705; EP 1242804 A2 20020925; JP 2004500562 A 20040108
DOCDB simple family (application)
US 0035543 W 20001229; AU 2606301 A 20001229; CA 2396015 A 20001229; EP 00989570 A 20001229; JP 2001548919 A 20001229