EP 1244782 A2 20021002 - METHOD FOR CARRYING OUT THE PARALLEL SEQUENCING OF A NUCLEIC ACID MIXTURE ON A SURFACE
Title (en)
METHOD FOR CARRYING OUT THE PARALLEL SEQUENCING OF A NUCLEIC ACID MIXTURE ON A SURFACE
Title (de)
VERFAHREN ZUR PARALLELEN SEQUENZIERUNG EINES NUKLEINSÄUREGEMISCHES AN EINER OBERFLÄCHE
Title (fr)
PROCEDE DE SEQUEN AGE PARALLELE D'UN MELANGE D'ACIDES NUCLEIQUES SUR UNE SURFACE
Publication
Application
Priority
- DE 19962893 A 19991223
- DE 10051564 A 20001018
- EP 0013157 W 20001222
Abstract (en)
[origin: WO0148184A2] The invention relates to a method for carrying out the parallel sequencing of at least two different nucleic acids contained in a nucleic acid mixture, whereby: (a) a surface is prepared which comprises islands of nucleic acids of the same type of tertiary nucleic acids; (b) opposite strands of the tertiary nucleic acids GTN are prepared; (c) the GTN are lengthened by one nucleotide, whereby the nucleotide, on the 2'-OH position or on the 3'-OH position, carries a protective group, which prevents an additional lengthening, and the nucleotide carries a molecular group, which enables the identification of the nucleotide; (d) the incorporated nucleotide is identified; (e) the protective group is removed and said molecular group of the incorporated nucleotide is removed or modified, and; (f) step (c) and the following steps are repeated until the desired sequence information has been obtained.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6874 (2013.01 - EP US)
C-Set (source: EP US)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0148184 A2 20010705; WO 0148184 A3 20020516; AU 3015801 A 20010709; EP 1244782 A2 20021002; US 2003186256 A1 20031002
DOCDB simple family (application)
EP 0013157 W 20001222; AU 3015801 A 20001222; EP 00990813 A 20001222; US 16855702 A 20020821