EP 1248680 A1 20021016 - APPARATUS AND METHOD FOR MIXING AND SEPARATION EMPLOYING MAGNETIC PARTICLES
Title (en)
APPARATUS AND METHOD FOR MIXING AND SEPARATION EMPLOYING MAGNETIC PARTICLES
Title (de)
APPARAT UND VERFAHREN ZUM MISCHEN UND TRENNEN UNTER BENÜTZUNG MAGNETISCHER TEILCHEN
Title (fr)
DISPOSITIF ET PROCEDE DE MELANGE ET DE SEPARATION AU MOYEN DE PARTICULES MAGNETIQUES
Publication
Application
Priority
US 0000071 W 20000104
Abstract (en)
[origin: WO0149419A1] An apparatus and method for carrying out the affinity separation of a target substance from a liquid test medium by mixing magnetic particles having surface immobilized ligand or receptor within the test medium to promote an affinity binding reaction between the ligand and the target substance. The test medium with the magnetic particles in a suitable container is removably mounted in an apparatus that creates a magnetic field gradient in the test medium. This magnetic gradient is used to induce the magnetic particles to move, thereby effecting mixing. The mixing is achieved either by movement of a magnet relative to a stationary container or movement of the container relative to a stationary magnet. In either case, the magnetic particles experience a continuous angular position change with the magnet. Concurrently with the relative angular movement between the magnet and the magnetic particles, the magnet is also moved along the length of the container causing the magnetic field gradient to sweep the entire length of the container. After the desired time, sufficient for the affinity reaction to occur, movement of the magnetic gradient is ended, whereby the magnetic particles are immobilized on the inside wall of the container nearest to the magnetic source. The remaining test medium is removed while the magnetic particles are retained on the wall of the container. The test medium or the particles may then be subjected to further processing.
IPC 1-7
IPC 8 full level
C12M 1/42 (2006.01); B03C 1/00 (2006.01); B03C 1/01 (2006.01); B03C 1/02 (2006.01); B03C 1/28 (2006.01)
CPC (source: EP)
B03C 1/01 (2013.01); B03C 1/288 (2013.01); B03C 2201/26 (2013.01)
Citation (search report)
See references of WO 0149419A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0149419 A1 20010712; AT E306328 T1 20051015; DE 60023185 D1 20060223; DE 60023185 T2 20060511; EP 1248680 A1 20021016; EP 1248680 B1 20051012; JP 2003519008 A 20030617; JP 4651894 B2 20110316
DOCDB simple family (application)
US 0000071 W 20000104; AT 00905525 T 20000104; DE 60023185 T 20000104; EP 00905525 A 20000104; JP 2001549777 A 20000104