EP 1259524 A2 20021127 - NUCLEOTIDES AND POLYNUCLEOTIDES IMMOBILISED ON A SOLID SUPPORT VIA A DISULPHIDE LINKAGE
Title (en)
NUCLEOTIDES AND POLYNUCLEOTIDES IMMOBILISED ON A SOLID SUPPORT VIA A DISULPHIDE LINKAGE
Title (de)
DURCH EINE DISULFIDBRÜCKE AUF EINEM FESTEN TRÄGER IMMOBILISIERTE NUKLEOTIDE UND POLYNUKLEOTIDE
Title (fr)
NUCLEOTIDES ET POLYNUCLEOTIDES IMMOBILISEES SUR UN SUPPORT SOLIDE AU MOYEN DE LIAISON DISULFURE
Publication
Application
Priority
- EP 00907844 A 20000307
- EP 99301764 A 19990309
- GB 0000843 W 20000307
Abstract (en)
[origin: WO0053616A2] A method of immobilising a nucleotide or polynucleotide on a solid support, comprises: providing the solid support having a functionalised surface of the formula B - S - S - L where B is the solid support and L is a leaving group; providing a nucleotide or polynucleotide comprising at least one phosphorothioate group; and coupling the two together by means of a sulphide exchange reaction. The product has the structure B - S - S - P(O)R<1>R<2>, where - POR<1>R<2> represents a nucleotide or a polynucleotide. Arrays of the immobilised polynucleotides are useful for performing APEX or SBH assays.
IPC 1-7
IPC 8 full level
C07H 19/10 (2006.01); C07H 19/20 (2006.01); C07H 21/00 (2006.01); C07H 21/04 (2006.01); C12M 1/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP)
C07H 19/10 (2013.01); C07H 19/20 (2013.01); C07H 21/00 (2013.01); G01N 33/54306 (2013.01)
Citation (search report)
See references of WO 0053616A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0053616 A2 20000914; WO 0053616 A3 20020912; AU 2930800 A 20000928; CA 2365226 A1 20000914; EP 1259524 A2 20021127; JP 2003520766 A 20030708
DOCDB simple family (application)
GB 0000843 W 20000307; AU 2930800 A 20000307; CA 2365226 A 20000307; EP 00907844 A 20000307; JP 2000604051 A 20000307