EP 1259644 A2 20021127 - NOVEL DNA CHIPS
Title (en)
NOVEL DNA CHIPS
Title (de)
NEUARTIGE DNA SONDEN
Title (fr)
NOUVELLES PUCES A ADN
Publication
Application
Priority
- FR 0100604 W 20010301
- FR 0002614 A 20000301
Abstract (en)
[origin: FR2805826A1] Detecting a mutation at a particular position in a target nucleic acid, comprising binding the target to a solid support, hybridizing a probe to the target, elongating the probe with nucleotide(s) resistant to exonuclease, digesting the probe with exonuclease and detecting bound nucleic acid, is new. Detecting a mutation in position 'n' in a target nucleic acid, comprising: (a) hybridizing a probe 5' bound to a DNA chip solid support with a target nucleic acid, where the 3' extremity of the probe maximally hybridizes up to the 'n' position; (b) elongating the hybridized probe by incorporating 5' to 3' nucleotides complementary to the target nucleic acid using a reaction mixture comprising at least one nucleotide derivative which is resistant to degradation with an exonuclease and a DNA polymerase; (c) digesting with the exonuclease so that only the non-elongated probes are digested; (d) washing; and (e) detecting the mutation by measuring indirectly or directly the presence of DNA. An Independent claim is also included for a device for carrying out the above method.
IPC 1-7
IPC 8 full level
C12M 1/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6827 (2018.01); C12Q 1/6837 (2018.01); G01N 21/64 (2006.01); G01N 27/04 (2006.01); G01N 27/06 (2006.01); G01N 27/327 (2006.01); G01N 27/414 (2006.01); G01N 27/416 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 37/00 (2006.01)
CPC (source: EP US)
C12Q 1/6827 (2013.01 - EP US); C12Q 1/6837 (2013.01 - EP US)
Citation (search report)
See references of WO 0164945A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
FR 2805826 A1 20010907; FR 2805826 B1 20020920; AU 4074301 A 20010912; CA 2401867 A1 20010907; EP 1259644 A2 20021127; JP 2003527846 A 20030924; US 2003186262 A1 20031002; WO 0164945 A2 20010907; WO 0164945 A3 20020328
DOCDB simple family (application)
FR 0002614 A 20000301; AU 4074301 A 20010301; CA 2401867 A 20010301; EP 01911814 A 20010301; FR 0100604 W 20010301; JP 2001563632 A 20010301; US 22050702 A 20021203