Global Patent Index - EP 1259644 A2

EP 1259644 A2 2002-11-27 - NOVEL DNA CHIPS

Title (en)

NOVEL DNA CHIPS

Title (de)

NEUARTIGE DNA SONDEN

Title (fr)

NOUVELLES PUCES A ADN

Publication

EP 1259644 A2 (FR)

Application

EP 01911814 A

Priority

  • FR 0100604 W
  • FR 0002614 A

Abstract (en)

[origin: FR2805826A1] Detecting a mutation at a particular position in a target nucleic acid, comprising binding the target to a solid support, hybridizing a probe to the target, elongating the probe with nucleotide(s) resistant to exonuclease, digesting the probe with exonuclease and detecting bound nucleic acid, is new. Detecting a mutation in position 'n' in a target nucleic acid, comprising: (a) hybridizing a probe 5' bound to a DNA chip solid support with a target nucleic acid, where the 3' extremity of the probe maximally hybridizes up to the 'n' position; (b) elongating the hybridized probe by incorporating 5' to 3' nucleotides complementary to the target nucleic acid using a reaction mixture comprising at least one nucleotide derivative which is resistant to degradation with an exonuclease and a DNA polymerase; (c) digesting with the exonuclease so that only the non-elongated probes are digested; (d) washing; and (e) detecting the mutation by measuring indirectly or directly the presence of DNA. An Independent claim is also included for a device for carrying out the above method.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

G01N 21/64 (2006.01); C12M 1/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 27/04 (2006.01); G01N 27/06 (2006.01); G01N 27/327 (2006.01); G01N 27/414 (2006.01); G01N 27/416 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 37/00 (2006.01)

CPC (invention and additional information)

C12Q 1/6827 (2013.01); C12Q 1/6837 (2013.01)

Combination set (CPC)

  1. C12Q 1/6827 + C12Q 2565/537 + C12Q 2533/101 + C12Q 2525/125
  2. C12Q 1/6827 + C12Q 2565/501 + C12Q 2533/101 + C12Q 2525/125
  3. C12Q 1/6837 + C12Q 2565/607 + C12Q 2565/537 + C12Q 2533/101

Citation (search report)

See references of WO 0164945A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

FR 2805826 A1 20010907; FR 2805826 B1 20020920; AU 4074301 A 20010912; CA 2401867 A1 20010907; EP 1259644 A2 20021127; JP 2003527846 A 20030924; US 2003186262 A1 20031002; WO 0164945 A2 20010907; WO 0164945 A3 20020328

INPADOC legal status


2005-04-13 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20041001

2002-11-27 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20020923

2002-11-27 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

2002-11-27 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI