EP 1259820 A2 20021127 - METHOD FOR ANALYSIS OF SUBSTANCES IN TISSUE OR IN CELLS
Title (en)
METHOD FOR ANALYSIS OF SUBSTANCES IN TISSUE OR IN CELLS
Title (de)
VERFAHREN ZUR ANALYSE VON SUBSTANZEN IN GEWEBE ODER IN ZELLEN
Title (fr)
PROCEDE D'ANALYSE DE SUBSTANCES PRESENTES DANS DES TISSUS OU CELLULES
Publication
Application
Priority
- JP 0101640 W 20010302
- JP 2000060754 A 20000306
- US 18675800 P 20000303
Abstract (en)
[origin: WO0165254A2] An improved method for directly identifying a chemical structure of a substance present in tissue or cells of organisms is disclosed. In particularly, the method for directly identifying a chemical structure of a substance present in tissue or cells of various kind of organisms comprises the following steps: (1) a step irradiating a laser to a certain intracellular region of a sample tissue slice or a cell, and analyzing the generating mass ions to obtain mass spectrum of the substance present at the cite, (2) a step analyzing the mass spectrum to obtain a mass profile of the substance existing at the intracellular region of the sample tissue slice or in cell, and(3) a step determining the chemical structure of the substance corresponding a certain molecular weight appeared in the mass profile. The method of this invention is conducted by utilizing the combined techniques of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and on-line capillary reversed-phase HPLC/quadrupole orthogonal acceleration time-of-flight (Q-Tof)-MS, and molecular cloning, is disclosed.
IPC 1-7
IPC 8 full level
G01N 33/68 (2006.01)
CPC (source: EP)
G01N 33/6818 (2013.01); G01N 33/6848 (2013.01); G01N 33/6851 (2013.01)
Citation (search report)
See references of WO 0165254A2
Citation (examination)
- NIESSEN W.M.A.: "State-of-the-art in liquid chromatography-mass spectrometry", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, vol. 856, no. 1-2, 24 September 1999 (1999-09-24), AMSTERDAM, NL, pages 179 - 197, XP004180079
- LEE M.S.; KERNS E.H.: "LC/MS applications in drug development", MASS SPECTROMETRY REVIEWS, vol. 18, 28 October 1999 (1999-10-28), pages 187 - 279
- ROURICK R.A. ET AL: "Predictive strategy for the rapid structure elucidation of drug degradants", JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol. 14, September 1996 (1996-09-01), pages 1743 - 1752
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0165254 A2 20010907; WO 0165254 A3 20020620; AU 3606401 A 20010912; EP 1259820 A2 20021127
DOCDB simple family (application)
JP 0101640 W 20010302; AU 3606401 A 20010302; EP 01908267 A 20010302