Global Patent Index - EP 1259820 A2

EP 1259820 A2 2002-11-27 - METHOD FOR ANALYSIS OF SUBSTANCES IN TISSUE OR IN CELLS

Title (en)

METHOD FOR ANALYSIS OF SUBSTANCES IN TISSUE OR IN CELLS

Title (de)

VERFAHREN ZUR ANALYSE VON SUBSTANZEN IN GEWEBE ODER IN ZELLEN

Title (fr)

PROCEDE D'ANALYSE DE SUBSTANCES PRESENTES DANS DES TISSUS OU CELLULES

Publication

EP 1259820 A2 (EN)

Application

EP 01908267 A

Priority

  • JP 0101640 W
  • JP 2000060754 A
  • US 18675800 P

Abstract (en)

[origin: WO0165254A2] An improved method for directly identifying a chemical structure of a substance present in tissue or cells of organisms is disclosed. In particularly, the method for directly identifying a chemical structure of a substance present in tissue or cells of various kind of organisms comprises the following steps: (1) a step irradiating a laser to a certain intracellular region of a sample tissue slice or a cell, and analyzing the generating mass ions to obtain mass spectrum of the substance present at the cite, (2) a step analyzing the mass spectrum to obtain a mass profile of the substance existing at the intracellular region of the sample tissue slice or in cell, and(3) a step determining the chemical structure of the substance corresponding a certain molecular weight appeared in the mass profile. The method of this invention is conducted by utilizing the combined techniques of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and on-line capillary reversed-phase HPLC/quadrupole orthogonal acceleration time-of-flight (Q-Tof)-MS, and molecular cloning, is disclosed.

IPC 1-7 (main, further and additional classification)

G01N 33/68

IPC 8 full level (invention and additional information)

G01N 33/68 (2006.01)

CPC (invention and additional information)

G01N 33/6848 (2013.01); G01N 33/6818 (2013.01); G01N 33/6851 (2013.01)

Citation (search report)

See references of WO 0165254A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

WO 0165254 A2 20010907; WO 0165254 A3 20020620; AU 3606401 A 20010912; EP 1259820 A2 20021127

INPADOC legal status


2008-01-02 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20070627

2007-03-21 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20050408

2005-05-25 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20050408

2002-11-27 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20020827

2002-11-27 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

2002-11-27 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Free text: AL;LT;LV;MK;RO;SI