EP 1265989 A2 20021218 - AN INFECTIVE ENDOGENOUS RETROVIRUS IN ASSOCIATION WITH DEMYELINATING DISEASES E.G. MULTIPLE SCLEROSIS
Title (en)
AN INFECTIVE ENDOGENOUS RETROVIRUS IN ASSOCIATION WITH DEMYELINATING DISEASES E.G. MULTIPLE SCLEROSIS
Title (de)
INFECTIÖSES ENDOGENES RETROVIRUS UND IHRE KORRELATION MIT DEMYELINIERENDER KRANKHEITEN Z.B. MULTIPEL SKLEROSE
Title (fr)
RETROVIRUS ENDOGENE INFECTIEUX, ET MALADIES ASSOCIEES DEMIELINISANTES ET AUTRES
Publication
Application
Priority
- DK PA200000466 A 20000321
- DK PA200000617 A 20000412
- EP 0103272 W 20010321
Abstract (en)
[origin: WO0170941A2] Retroviral sequences were characterised by RT-PCR with <i>gag</i> and <i>env</i> primers on RNA from RT-positive retroviral particles produced by multiple sclerosis (MS) derived B-lymphoblastoid cell lines. Sequence variants with high homology to the potentially functional subgroup RGH of the human endogenous retrovirus RTVL-H/HERV-H family were found. The same sequences were also specifically found in the particulate fraction of a series of MS patient plasma samples and were absent in controls. South-Western blots demonstrated the presence of a nucleic acid binding protein, corresponding in size and function to the nucleocapsid protein, Gag NC, of other retroviruses. Indications for transmission of the retrovirus to mononuclear blood cells from healthy human individuals and non-human animals were found. Cell cultures derived from peripheral blood from MS patients contained 5-15 % CD3+ T-cells and a specific splice variant of the <i>env</i> sequence comprising a region of the <i>pol</i> region was identified in such cultures.
[origin: WO0170941A2] Retroviral sequences were characterised by RT-PCR with gag and env primers on RNA from RT-positive retroviral particles produced by multiple sclerosis (MS) derived B-lymphoblastoid cell lines. Sequence variants with high homology to the potentially functional subgroup RGH of the human endogenous retrovirus RTVL-H/HERV-H family were found. The same sequences were also specifically found in the particulate fraction of a series of MS patient plasma samples and were absent in controls. South-Western blots demonstrated the presence of a nucleic acid binding protein, corresponding in size and function to the nucleocapsid protein, Gag NC, of other retroviruses. Indications for transmission of the retrovirus to mononuclear blood cells from healthy human individuals and non-human animals were found. Cell cultures derived from peripheral blood from MS patients contained 5-15 % CD3+ T-cells and a specific splice variant of the env sequence comprising a region of the pol region was identified in such cultures.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); A61K 31/7088 (2006.01); A61K 38/43 (2006.01); A61K 39/395 (2006.01); A61K 45/00 (2006.01); A61K 48/00 (2006.01); A61P 3/10 (2006.01); A61P 25/00 (2006.01); A61P 31/18 (2006.01); A61P 35/00 (2006.01); A61P 37/02 (2006.01); C07K 14/15 (2006.01); C12N 5/07 (2010.01); C12N 5/09 (2010.01); C12N 7/00 (2006.01); C12Q 1/02 (2006.01); C12Q 1/68 (2006.01); C12Q 1/70 (2006.01); A61K 38/00 (2006.01)
CPC (source: EP)
A61P 3/10 (2017.12); A61P 25/00 (2017.12); A61P 31/18 (2017.12); A61P 35/00 (2017.12); A61P 37/02 (2017.12); C07K 14/005 (2013.01); C12N 7/00 (2013.01); C12Q 1/70 (2013.01); A61K 38/00 (2013.01); C12N 2740/10021 (2013.01); C12N 2740/10022 (2013.01)
Citation (search report)
See references of WO 0170941A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0170941 A2 20010927; WO 0170941 A3 20020808; WO 0170941 A9 20030522; AU 5829201 A 20011003; CA 2403815 A1 20010927; EP 1265989 A2 20021218; IL 151868 A0 20030410; JP 2004502407 A 20040129
DOCDB simple family (application)
EP 0103272 W 20010321; AU 5829201 A 20010321; CA 2403815 A 20010321; EP 01931543 A 20010321; IL 15186801 A 20010321; JP 2001569324 A 20010321