EP 1266202 A4 20051109 - METHODS FOR DETECTION OF DIFFERENCES IN NUCLEIC ACIDS
Title (en)
METHODS FOR DETECTION OF DIFFERENCES IN NUCLEIC ACIDS
Title (de)
VERFAHREN ZUR ERKENNUNG VON UNTERSCHIEDEN IN NUKLEINSAÜREN
Title (fr)
METHODES DE DETECTION DES DIFFERENCES ENTRE ACIDES NUCLEIQUES
Publication
Application
Priority
- US 0107858 W 20010312
- US 18866900 P 20000311
- US 22888500 P 20000829
- US 23422900 P 20000921
- US 23436300 P 20000922
- US 24277000 P 20001023
- US 24284000 P 20001023
Abstract (en)
[origin: WO0169200A2] The present invention provides methods for detecting the presence or absence of a difference between two related nucleic acid sequences. In the methods, a target nucleic acid and a reference nucleic acid are contacted under conditions in which they are capable of forming a four-way nucleic acid complex with a branch structure that is capable of migration. Under the contact conditions, if the reference nucleic acid and target nucleic acid are identical, branch migration is capable of going to completion resulting in complete strand exchange. If the reference nucleic acid and target nucleic acid are different, branch migration does not go to completion, resulting in a stable four-way complex. Detection of the stable four-way complex identifies the presence of a difference between the nucleic acids. A stable four-way complex can be detected with molecules that specifically bind such complexes, by gel electrophoresis or by specific isolation of the stable four-way complex.
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); C12N 15/09 (2006.01); C12Q 1/34 (2006.01); C12Q 1/68 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP)
C12Q 1/6827 (2013.01)
C-Set (source: EP)
Citation (search report)
- [X] WO 9723646 A1 19970703 - BEHRINGWERKE AG [DE], et al
- [X] WO 9936574 A1 19990722 - MAYO FOUNDATION [US]
- [A] DAVIES ADELINA A ET AL: "Formation of RuvABC-Holliday junction complexes in vitro", CURRENT BIOLOGY, vol. 8, no. 12, 4 June 1998 (1998-06-04), pages 725 - 727, XP002339439, ISSN: 0960-9822
- [A] BOLT E L ET AL: "Identification of three aspartic acid residues essential for catalysis by the RusA holliday junction resolvase", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 286, no. 2, 19 February 1999 (1999-02-19), pages 403 - 415, XP004459761, ISSN: 0022-2836
- [A] ICHIYANAGI KENJI ET AL: "Mutational analysis on structure-function relationship of a Holliday junction specific endonuclease RuvC", GENES TO CELLS, vol. 3, no. 9, September 1998 (1998-09-01), pages 575 - 586, XP002339440, ISSN: 1356-9597
- [A] FRAPPIER L ET AL: "CHARACTERIZATION OF THE BINDING SPECIFICITY OF TWO ANTICRUCIFORM DNA MONOCLONAL ANTIBODIES", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 1, 1989, pages 334 - 341, XP002339441, ISSN: 0021-9258
- [A] BHATTACHARYYA A ET AL: "MODEL FOR THE INTERACTION OF DNA JUNCTIONS AND RESOLVING ENZYMES", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 221, no. 4, 1991, pages 1191 - 1207, XP000612384, ISSN: 0022-2836
- See references of WO 0169200A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0169200 A2 20010920; WO 0169200 A3 20020314; AU 2001245634 B2 20051006; AU 4563401 A 20010924; CA 2402483 A1 20010920; CN 1191374 C 20050302; CN 1429277 A 20030709; EP 1266202 A2 20021218; EP 1266202 A4 20051109; JP 2003527119 A 20030916
DOCDB simple family (application)
US 0107858 W 20010312; AU 2001245634 A 20010312; AU 4563401 A 20010312; CA 2402483 A 20010312; CN 01809310 A 20010312; EP 01918574 A 20010312; JP 2001568034 A 20010312