EP 1272624 A1 20030108 - A PROCESS FOR THE PRODUCTION OF HUMAN INTERFERON ALPHA FROM GENETICALLY ENGINEERED YEAST
Title (en)
A PROCESS FOR THE PRODUCTION OF HUMAN INTERFERON ALPHA FROM GENETICALLY ENGINEERED YEAST
Title (de)
VERFAHREN ZUR HERSTELLUNG VON HUMANEM INTERFERON ALPHA AUS GENETISCH VERÄNDERTER HEFE
Title (fr)
PROCEDE PERMETTANT DE PRODUIRE DE L'INTERFERON ALPHA HUMAIN A PARTIR DE LEVURE GENETIQUEMENT MODIFIEE
Publication
Application
Priority
IB 0000339 W 20000316
Abstract (en)
[origin: WO0168827A1] A process for the production of physiologically-active human interferon alpha from genetically engineered yeast, <i>Pichia pastoris</i>, comprising digesting, with an enzyme to produce a linearized plasmid, a plasmid having a promoter and operationally linked to a human interferon alpha gene in the absence of a fusion region; transforming <i>Pichia pastoris</i> cells with the linearized plasmid by homologous recombination to form <i>Pichia pastoris</i> clones; screening the <i>Pichia pastoris</i> clones for high interferon alpha expression to find a high interferon-yielding <i>Pichia pastoris</i> clone; growing the high interferon-yielding <i>Pichia pastoris</i> clone; and purifying physiologically-active human interferon alpha protein from the high interferon-yielding <i>Pichia pastoris</i> clones.
IPC 1-7
C12N 15/00; C12N 5/10; C12N 1/15; C12N 1/16; C12P 21/02; C07K 14/47; C07K 14/555
IPC 8 full level
C12N 15/09 (2006.01); C07K 14/47 (2006.01); C07K 14/555 (2006.01); C07K 14/56 (2006.01); C12N 1/15 (2006.01); C12N 1/16 (2006.01); C12N 5/10 (2006.01); C12N 15/00 (2006.01); C12P 21/02 (2006.01); C12R 1/84 (2006.01)
CPC (source: EP)
C07K 14/56 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0168827 A1 20010920; AU 3186300 A 20010924; CA 2402562 A1 20010920; CN 1452658 A 20031029; EP 1272624 A1 20030108; EP 1272624 A4 20040616; JP 2003526365 A 20030909
DOCDB simple family (application)
IB 0000339 W 20000316; AU 3186300 A 20000316; CA 2402562 A 20000316; CN 00819549 A 20000316; EP 00909584 A 20000316; JP 2001567311 A 20000316