EP 1277050 A2 20030122 - METHOD OF DETERMINING THE THREE-DIMENSIONAL SHAPE OF A MACROMOLECULE
Title (en)
METHOD OF DETERMINING THE THREE-DIMENSIONAL SHAPE OF A MACROMOLECULE
Title (de)
VERFAHREN ZUR BESTIMMUNG DER DREIDIMENSIONALEN GESTALT EINES MAKROMOLEKÜLES
Title (fr)
PROCEDE DE DETERMINATION DE LA FORME TRIDIMENSIONNELLE D'UNE MACROMOLECULE
Publication
Application
Priority
- US 0014667 W 20000526
- US 13589199 P 19990526
Abstract (en)
[origin: WO0072004A2] The present invention provides a fast and efficient method for determining the three-dimensional conformation of a protein. The steps of the method of the invention include: 1) formation of physical distance constraints, e.g., forming intramolecular chemical crosslinks of known size between residues of a protein; 2) enriching the number of the molecules that have intramolecular chemical crosslinks in the reaction pool, e.g., using size separation to remove proteins with intermolecular bonds; 3) exposing the enriched reaction pool to a protease that cuts the protein at specific sites to produce peptide fragments; 4) measuring the size of the peptide fragments to determine linkage sites with a certain spatial relationship in the protein; and 5) interpreting the data produced to determine spatial geometry and protein structure based on the deduced spatial relationship of the linkage sites. The information is preferably analyzed with aid from a computer system, which can be used to generate and/or analyze distance constraints between amino acids.
IPC 1-7
IPC 8 full level
G01N 27/62 (2006.01); B01J 20/283 (2006.01); C12N 15/09 (2006.01); G01N 30/06 (2006.01); G01N 30/26 (2006.01); G01N 30/72 (2006.01); G01N 30/84 (2006.01); G01N 30/88 (2006.01); G01N 33/48 (2006.01); G01N 33/68 (2006.01); G16B 15/20 (2019.01); H01J 49/04 (2006.01)
CPC (source: EP US)
G01N 33/6818 (2013.01 - EP); G01N 33/6842 (2013.01 - EP); G01N 33/6848 (2013.01 - EP); G01N 33/6851 (2013.01 - EP); G16B 15/20 (2019.01 - EP US); H01J 49/04 (2013.01 - EP); G16B 15/00 (2019.01 - EP)
Citation (search report)
See references of WO 0072004A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0072004 A2 20001130; WO 0072004 A3 20021128; WO 0072004 A9 20020829; AU 5298900 A 20001212; EP 1277050 A2 20030122; JP 2003528288 A 20030924
DOCDB simple family (application)
US 0014667 W 20000526; AU 5298900 A 20000526; EP 00937870 A 20000526; JP 2000620343 A 20000526