EP 1278773 A2 20030129 - SELECTABLE CELL SURFACE MARKER GENES
Title (en)
SELECTABLE CELL SURFACE MARKER GENES
Title (de)
SELEKTIERBARE ZELLOBERFLÄCHENMARKERGENEN
Title (fr)
GENES MARQUEURS DE SURFACE SELECTIONNABLES
Publication
Application
Priority
- EP 0011474 W 20001117
- US 16659499 P 19991119
- US 44403899 A 19991119
- US 53924800 A 20000330
Abstract (en)
[origin: WO0136659A2] The present invention concerns a method of identifying genetically modified mammalian cells using a mutated protein-tyrosine kinase receptor (PTKR) as a selectable marker in mammalian cells. Particularly preferred mutated PTKR selective markers are mutated epidermal growth factor receptor (EGFR) family members, and muscle specific tyrosine kinase receptor (MuSK-R) family members. Further a method for the immunoselection of transduced mammalian cells is disclosed comprising retrovirally transducing mammalian cells with a nucleic acid sequence encoding a mutated EGFR, incubating the transduced cells with a marked antibody which recognizes and binds specifically to the mutated PTKR, and identifying the marked transduced cells.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C07K 14/705 (2006.01); C12N 9/12 (2006.01); C12N 15/09 (2006.01); C12Q 1/02 (2006.01); G01N 33/15 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 33/569 (2006.01)
CPC (source: EP)
C07K 14/705 (2013.01); C12N 9/12 (2013.01); C12N 9/1205 (2013.01); G01N 33/56966 (2013.01)
Citation (search report)
See references of WO 0136659A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0136659 A2 20010525; WO 0136659 A3 20021114; AU 2507001 A 20010530; AU 779462 B2 20050127; CA 2391593 A1 20010525; EP 1278773 A2 20030129; IL 149621 A0 20021110; JP 2003522525 A 20030729; NZ 518877 A 20040326
DOCDB simple family (application)
EP 0011474 W 20001117; AU 2507001 A 20001117; CA 2391593 A 20001117; EP 00988723 A 20001117; IL 14962100 A 20001117; JP 2001538537 A 20001117; NZ 51887700 A 20001117