EP 1278832 A1 20030129 - METHODS AND COMPOSITIONS FOR EFFECTING HOMOLOGOUS RECOMBINATION
Title (en)
METHODS AND COMPOSITIONS FOR EFFECTING HOMOLOGOUS RECOMBINATION
Title (de)
METHODEN UND ZUSAMMENSETZUNGEN ZUR WIRKUNGSVOLLEN HOMOLOGEN REKOMBINATION
Title (fr)
METHODES ET COMPOSITIONS DE REALISATION DE RECOMBINAISON HOMOLOGUE
Publication
Application
Priority
- EP 01925779 A 20010503
- EP 00401218 A 20000503
- IB 0100749 W 20010503
Abstract (en)
[origin: EP1152058A1] This invention concerns a method for effecting homologous recombination between a native nucleic acid segment in a cell and a donor nucleic acid segment introduced into the cell, which comprises : a) introducing into a cell a nucleic acid delivery system comprising: (i) an oligonucleotide third strand which comprises a base sequence capable of forming a triple helix at a binding region on one or both strands of a native nucleic acid segment, (ii) a donor nucleic acid, comprising a nucleic acid sequence sufficiently homologous to the native nucleic acid segment such that the donor sequence is capable of undergoing homologous recombination with the native sequence at the target region, (iii) an adapter segment comprising an oligonucleotide sequence able to bind at least a portion of said donor nucleic acid through Watson-Crick base pairing, the adapter segment being linked to said oligonucleotide third strand, b) allowing the oligonucleotide to bind to the native nucleic acid segment to form a triple helix nucleic acid, thereby inducing homologous recombination at the native nucleic acid segment target region ; and c) allowing homologous recombination to occur between the native and donor nucleic acid segments. The method may namely be implemented for effecting gene alteration or mutation repair at a specific sequence site on a native DNA. <IMAGE>
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); A61K 48/00 (2006.01); C07H 21/04 (2006.01); C12N 15/10 (2006.01); C12N 15/90 (2006.01)
CPC (source: EP US)
C12N 15/102 (2013.01 - EP US); C12N 15/902 (2013.01 - EP US)
Citation (search report)
See references of WO 0183735A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
EP 1152058 A1 20011107; AU 5245901 A 20011112; CA 2407900 A1 20011108; EP 1278832 A1 20030129; JP 2003531610 A 20031028; US 2003003547 A1 20030102; US 6936418 B2 20050830; WO 0183735 A1 20011108
DOCDB simple family (application)
EP 00401218 A 20000503; AU 5245901 A 20010503; CA 2407900 A 20010503; EP 01925779 A 20010503; IB 0100749 W 20010503; JP 2001580343 A 20010503; US 5352602 A 20020118