EP 1278892 A1 20030129 - DETECTION OF VARIATIONS IN THE DNA METHYLATION PROFILE
Title (en)
DETECTION OF VARIATIONS IN THE DNA METHYLATION PROFILE
Title (de)
DETEKTION VON VARIATIONEN DES DNA-METHYLIERUNGSPROFILS
Title (fr)
DETECTION DE VARIATIONS DANS LE PROFIL DE METHYLATION DE L'ADN
Publication
Application
Priority
- DE 0101486 W 20010406
- DE 10019058 A 20000406
Abstract (en)
[origin: DE10019058A1] A nucleic acid, (I), comprising a sequence of 18 bases, of a segment of chemically pretreated DNA (CP DNA) of genes associated with cell cycle having a sequence taken from 424 sequences (Ss) and sequences complementary to (Ss), is new. Independent claims are also included for the following: (1) an oligomer (II), particularly an oligonucleotide or peptide nucleic acid (PNA)-oligomer, comprising a base sequence having a length of at least 9 nucleotides which hybridizes to or is identical to a CP DNA of genes associated with the cell cycle having a sequence from (Ss); (2) a set of (II), comprising at least two (II); (3) use of a set of oligomer probes (III) for detecting the cytosine methylation state and/or single nucleotide polymorphisms (SNPs) in CP DNA; (4) manufacturing an arrangement of different oligomers (array) fixed to a carrier material for analyzing diseases associated with the methylation state of the CpG dinucleotides of CP DNA, where at least a (II) is coupled to a solid phase; (5) an arrangement of different oligomers (array), obtained by the method (4); (6) ascertaining (M1) genetic and/or epigenetic parameters for the diagnosis and/or therapy of existing diseases or the predisposition to specific diseases by analyzing cytosine methylations, comprising: (a) converting cytosine bases which are unmethylated at the 5-position (in a genomic DNA sample) to uracil or another base which is dissimilar to cytosine in terms of hybridization behavior, by chemical treatment; (b) amplifying fragments of the chemically pretreated DNA using sets of (II) as primers and a polymerase, the amplifies carrying a detectable label; (c) hybridizing the amplificate to a set of (II) or else to a DNA-and/or PNA-array; and (d) detecting the hybridized amplifies; and (7) a kit (IV) comprising a bisulfite (= disulfite, hydrogen sulfite) reagent as well as DNA- and/or PNA-oligomers. - ACTIVITY : Anti-HIV; neuroprotective; immunosuppressive; antitumor; cytostatic; antiarthritic; antiarteriosclerotic. - MECHANISM OF ACTION : Cytosine methylation regulator. No example is given.
IPC 1-7
IPC 8 full level
C07K 14/47 (2006.01); C07K 14/82 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6883 (2018.01)
CPC (source: EP US)
C07K 14/4703 (2013.01 - EP US); C07K 14/82 (2013.01 - EP US); C12Q 1/6883 (2013.01 - EP US); C12Q 2600/154 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
DE 10019058 A1 20011220; AU 7384001 A 20011023; EP 1278892 A1 20030129; US 2007026393 A1 20070201; WO 0177373 A2 20011018
DOCDB simple family (application)
DE 10019058 A 20000406; AU 7384001 A 20010406; DE 0101486 W 20010406; EP 01940158 A 20010406; US 24097003 A 20030711