EP 1280465 A4 20041020 - MICROFLUIDIC CHANNEL EMBRYO AND/OR OOCYTE HANDLING, ANALYSIS AND BIOLOGICAL EVALUATION
Title (en)
MICROFLUIDIC CHANNEL EMBRYO AND/OR OOCYTE HANDLING, ANALYSIS AND BIOLOGICAL EVALUATION
Title (de)
BIOLOGISCHE EVALUIERUNG, ANALYSE UND MANIPULATION VON EMBRYOS UND/ODER OVOZYTEN IN EINEM MIKROFLÜSSIGKEITSKANAL
Title (fr)
EVALUATION BIOLOGIQUE, ANALYSE ET MANIPULATION D'OVOCYTES ET/OU D'EMBRYONS DANS UN CANAL MICROFLUIDIQUE
Publication
Application
Priority
- US 0114822 W 20010508
- US 57048300 A 20000512
Abstract (en)
[origin: WO0188087A2] Microfluidic embryo scaled channels (14) for handling and positioning embryos provide the opportunity to evaluate and treat embryos in improved manners. Fluid flow is used to move and position embryos within microfluidic channels and channel geometrics may be used to place embryos at specific locations. Surface properties and compliance (deformation) properties of embryos are evaluated as a predictor of viability. The microfluidic channels provide the opportunity for fine controls of pressure to conduct various evalutions at forces slightly below which damage to embryos is known to occur. Measurement of the distance and/or which embryos roll in a same pressure gradient microfluidic channel provides information, with healthy embryos traveling slower or a shorter distance as they demonstrate more stiction to channel walls. Positioned at a constriction (14a, 14b, 24, 26), health embryos also appear to deform less than unhealthy embryos that are more readily pulled into a constriction. In addition, healthy embryos appear to resume their shape better. Fluid from microfluidic channels is easily collected downstream without altering the embryo environment, providing a better opportunity for chemical analysis of fluid chemical analysis than convention manual handling and sampling techniques. Zona pellucida removal of mammalian embryos is achieved as embryos are moved through flow to a precise location where lysing agent can be washed over the embryo to achieve zona removal. Cumulus removal is realized with a series of constrictions to cut cumulus followed by fluid flows to remove cut cumulus from the embryo.
IPC 1-7
IPC 8 full level
A01K 67/02 (2006.01); A61B 17/43 (2006.01); B81B 1/00 (2006.01); C12M 1/00 (2006.01); C12Q 1/02 (2006.01); G01N 33/48 (2006.01)
CPC (source: EP KR)
B01L 3/502746 (2013.01 - EP); B01L 3/502761 (2013.01 - EP); B01L 3/502776 (2013.01 - EP); C12M 21/06 (2013.01 - EP); C12M 23/16 (2013.01 - EP); C12Q 1/00 (2013.01 - KR); B01L 2200/0636 (2013.01 - EP); B01L 2200/0668 (2013.01 - EP); B01L 2300/0816 (2013.01 - EP); B01L 2400/0418 (2013.01 - EP); B01L 2400/0487 (2013.01 - EP); B01L 2400/086 (2013.01 - EP)
Citation (search report)
- [A] WO 9961888 A2 19991202 - CALIFORNIA INST OF TECHN [US], et al
- [XY] I.K. GLASGOW, H.C. ZERINGUE, D.J. BEEBE, S.J. CHOI, J.T.LYMAN, M.B. WHEELER: "Individual Embryo Transport and Retention on a Chip", MICRO TOTAL ANALYSIS SYSTEMS 98, PROCEEDINGS OF THE TAS 98, WORKSHOP HELD IN BANFF, CANADA, D.J. HARRISON AND A.VAN DEN BERG, 1998, pages 199 - 202, XP009035459
- [Y] S.J. CHOI ET AL.: "Development of Microelectrmechanicla Systems to analyze individual mammalian embryos: embryo biocompatibility", BIOL. REPROD. , SUPPL. 1, vol. 58, 1998, pages 96, XP009035449
- [A] WHEELER ET AL.: "Developement of microelectromechanical systems to analyze individual mammalian embryos: embryo biocompatability and individual embryo transport on silicon chip", ARQ. FAC. VET. UFRGS, vol. 26, no. 1, 1998, PORTO ALEGRE, pages 391, XP001183069
- [A] CHAN ET AL.: "Development of an Embryo Transport and Analysis System: Material Biocompatibility", THERIOGENOLOGY, vol. 51, 1999, pages 234, XP001183416
- See references of WO 0188087A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0188087 A2 20011122; WO 0188087 A3 20020328; AU 2001259635 B2 20050519; AU 5963501 A 20011126; BR 0110768 A 20030311; CA 2408616 A1 20011122; CA 2408616 C 20071002; CN 1441652 A 20030910; EP 1280465 A2 20030205; EP 1280465 A4 20041020; IL 152650 A0 20030624; IL 152650 A 20061231; JP 2003533221 A 20031111; KR 20030009482 A 20030129; MX PA02011150 A 20040819; RU 2002130577 A 20040327
DOCDB simple family (application)
US 0114822 W 20010508; AU 2001259635 A 20010508; AU 5963501 A 20010508; BR 0110768 A 20010508; CA 2408616 A 20010508; CN 01812654 A 20010508; EP 01933192 A 20010508; IL 15265001 A 20010508; JP 2001585296 A 20010508; KR 20027015046 A 20021109; MX PA02011150 A 20010508; RU 2002130577 A 20010508