EP 1284421 A1 20030219 - METHOD OF CONSTRUCTING POLYNUCLEOTIDE MICROARRAY, APPARATUS FOR THE CONSTRUCTION AND POLYNUCLEOTIDE MICROARRAY
Title (en)
METHOD OF CONSTRUCTING POLYNUCLEOTIDE MICROARRAY, APPARATUS FOR THE CONSTRUCTION AND POLYNUCLEOTIDE MICROARRAY
Title (de)
VERFAHREN ZUM HERSTELLEN VON POLYNUKLEOTIDMICROPROTEINANORDNUNGEN, UND VORRICHTUNG FÜR IHRE HERSTELLUNG
Title (fr)
MICRORESEAU DE POLYNUCLEOTIDES, PROCEDE ET APPAREIL PERMETTANT LA CONSTRUCTION DE CELUI-CI
Publication
Application
Priority
- JP 0103899 W 20010510
- JP 2000139926 A 20000512
Abstract (en)
An object of the present invention is to provide a method for preparing a polynucleotide microarray which enables fixation of a sufficient amount of polynucleotides onto the support and can provide signals having uniform and sufficient intensity within a spot area, even when the polynucleotides are synthetic oligo polynucleotides which have a short chain length and are known to be difficult to be fixed by electrostatic binding. The present invention provides a method for preparing a polynucleotide microarray, wherein a solution for dissolving polynucleotides which is used to fix the polynucleotides onto a support contains substantially no anionic electrolyte, and is a solution of water or mixture of water and an organic solvent; and when the solution is a mixture of water and an organic solvent, the content of the organic solvent in the solution is less than 50 volume %.
IPC 1-7
IPC 8 full level
B01J 19/00 (2006.01); C07H 21/00 (2006.01); C07H 21/04 (2006.01); C12Q 1/68 (2006.01); C40B 60/14 (2006.01); G01N 33/52 (2006.01); G01N 33/543 (2006.01); C40B 40/06 (2006.01)
CPC (source: EP US)
B01J 19/0046 (2013.01 - EP US); B82Y 30/00 (2013.01 - EP US); C07H 21/00 (2013.01 - EP US); C07H 21/04 (2013.01 - EP US); C12Q 1/6837 (2013.01 - EP US); C40B 40/06 (2013.01 - EP US); C40B 50/14 (2013.01 - EP US); C40B 60/14 (2013.01 - EP US); G01N 33/52 (2013.01 - EP US); G01N 33/54393 (2013.01 - EP US); B01J 2219/00382 (2013.01 - EP US); B01J 2219/00387 (2013.01 - EP US); B01J 2219/00441 (2013.01 - EP US); B01J 2219/00527 (2013.01 - EP US); B01J 2219/00585 (2013.01 - EP US); B01J 2219/00596 (2013.01 - EP US); B01J 2219/00605 (2013.01 - EP US); B01J 2219/00608 (2013.01 - EP US); B01J 2219/00612 (2013.01 - EP US); B01J 2219/00617 (2013.01 - EP US); B01J 2219/00626 (2013.01 - EP US); B01J 2219/00659 (2013.01 - EP US); B01J 2219/00677 (2013.01 - EP US); B01J 2219/00707 (2013.01 - EP US); B01J 2219/00711 (2013.01 - EP US); B01J 2219/00722 (2013.01 - EP US); C07B 2200/11 (2013.01 - EP US); C12Q 2565/518 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
EP 1284421 A1 20030219; EP 1284421 A4 20051228; AU 5668801 A 20011120; CA 2408767 A1 20011115; JP 4728555 B2 20110720; JP WO2001086294 A1 20040108; US 2004091867 A1 20040513; WO 0186294 A1 20011115
DOCDB simple family (application)
EP 01930028 A 20010510; AU 5668801 A 20010510; CA 2408767 A 20010510; JP 0103899 W 20010510; JP 2001583187 A 20010510; US 27527303 A 20030702