Global Patent Index - EP 1287154 A4

EP 1287154 A4 20101215 - CHARGE-SWITCH NUCLEOTIDES

Title (en)

CHARGE-SWITCH NUCLEOTIDES

Title (de)

LADUNGSWECHSEL-NUKLEOTIDE

Title (fr)

NUCLEOTIDES A COMMUTATION DE CHARGES

Publication

EP 1287154 A4 20101215 (EN)

Application

EP 01946213 A 20010607

Priority

  • US 0118699 W 20010607
  • US 20989600 P 20000607
  • US 28623801 P 20010424
  • US 87637401 A 20010606
  • US 87637501 A 20010606

Abstract (en)

[origin: WO0194609A1] The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.

IPC 8 full level

C07H 19/10 (2006.01); G01N 21/64 (2006.01); C07H 19/20 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/00 (2006.01); C12Q 1/42 (2006.01); C12Q 1/44 (2006.01); C12Q 1/48 (2006.01); C12Q 1/68 (2006.01); G01N 27/447 (2006.01); G01N 33/483 (2006.01); G01N 33/53 (2006.01); C07B 61/00 (2006.01)

CPC (source: EP)

C07H 19/10 (2013.01); C07H 19/20 (2013.01); C07H 21/00 (2013.01); C12Q 1/6869 (2013.01); C40B 40/00 (2013.01)

C-Set (source: EP)

C12Q 1/6869 + C12Q 2565/629 + C12Q 2565/607 + C12Q 2565/301

Citation (search report)

  • [X] US 5260433 A 19931109 - ENGELHARDT DEAN [US], et al
  • [A] US 5302509 A 19940412 - CHEESEMAN PETER C [US]
  • [A] EP 0967219 A1 19991229 - RIKAGAKU KENKYUSHO [JP], et al
  • [A] WO 9700967 A1 19970109 - BAYLOR COLLEGE MEDICINE [US]
  • [X] K. C. TSOU ET AL.: "Indigogenic Phosphodiesters as Potential Chromogenic Cancer Chemotherapeutic Agents", JOURNAL OF MEDICIANL CHEMISTRY, vol. 25, no. 12, 1972, pages 1221 - 1225, XP002591210
  • [A] RONAGHI ET AL: "A SEQUENCING METHOD BASED ON REAL-TIME PYROPHOSPHATE", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, WASHINGTON, DC; US LNKD- DOI:10.1126/SCIENCE.281.5375.363, vol. 281, 17 July 1998 (1998-07-17), pages 363 - 365, XP002135869, ISSN: 0036-8075
  • [A] GOODWIN P M ET AL: "APPLICATION OF SINGLE MOLECULE DETECTION TO DNA SEQUENCING", NUCLEOSIDES & NUCLEOTIDES, MARCEL DEKKER, INC, US, vol. 16, no. 5/06, 1 January 1997 (1997-01-01), pages 543 - 550, XP001109270, ISSN: 0732-8311
  • See references of WO 0194609A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

DOCDB simple family (publication)

WO 0194609 A1 20011213; AU 6829101 A 20011217; CA 2412567 A1 20011213; EP 1287154 A1 20030305; EP 1287154 A4 20101215; EP 2226330 A1 20100908; EP 2226330 B1 20130918; JP 2004516810 A 20040610

DOCDB simple family (application)

US 0118699 W 20010607; AU 6829101 A 20010607; CA 2412567 A 20010607; EP 01946213 A 20010607; EP 09008795 A 20010607; JP 2002502149 A 20010607