Global Patent Index - EP 1294869 A2

EP 1294869 A2 20030326 - WHOLE CELL ENGINEERING BY MUTAGENIZING A SUBSTANTIAL PORTION OF A STARTING GENOME, COMBINING MUTATIONS, AND OPTIONALLY REPEATING

Title (en)

WHOLE CELL ENGINEERING BY MUTAGENIZING A SUBSTANTIAL PORTION OF A STARTING GENOME, COMBINING MUTATIONS, AND OPTIONALLY REPEATING

Title (de)

MANIPULATION VON GANZEN ZELLEN DURCH MUTATION EINES GROSSTEILS DES STARGENOMS, KOMBINATION DER MUTATIONEN UND WAHLWEISES WIEDERHOLEN

Title (fr)

INGENIERIE CELLULAIRE COMPLETE PAR MUTAGENESE D'UNE PARTIE SUBSTANTIELLE D'UN GENOME DE DEPART, PAR COMBINAISON DE MUTATIONS ET EVENTUELLEMENT REPETITION

Publication

EP 1294869 A2 20030326 (EN)

Application

EP 01944583 A 20010614

Priority

  • US 67758400 A 20000930
  • US 0119367 W 20010614
  • US 59445900 A 20000614

Abstract (en)

[origin: WO0196551A2] An invention comprising cellular transformation, directed evolution, and screening methods for creating novel transgenic organisms having desirable properties. Thus in one aspect, this invention relates to a method of generating a transgenic organism, such as a microbe or a plant, having a plurality of traits that are differentially activatable. Also, a method of retooling genes and gene pathways by the introduction of regulatory sequences, such as promoters, that are operable in an intended host, thus conferring operability to a novel gene pathway when it is introduced into an intended host. For example a novel man-made gene pathway, generated based on microbially-derived progenitor templates, that is operable in a plant cell. Furthermore, a method of generating novel host organisms having increased expression of desirable traits, recombinant genes, and gene products.

IPC 1-7

C12N 15/10; C12Q 1/68

IPC 8 full level

C12N 15/10 (2006.01); C12N 15/82 (2006.01); G01N 33/534 (2006.01); G01N 33/68 (2006.01)

CPC (source: EP)

C12N 15/102 (2013.01); C12N 15/1027 (2013.01); C12N 15/1058 (2013.01); C12N 15/8241 (2013.01); G01N 33/534 (2013.01); G01N 33/6818 (2013.01)

Citation (search report)

See references of WO 0196551A2

Citation (examination)

  • WO 0009755 A2 20000224 - PANGENE CORPORATION [US]
  • WO 0009679 A1 20000224 - PROTEUS SA [FR], et al
  • BENDER AND KLECKNER: "TN10 insertion specificity is strongly dependent upon sequences immediately adjacent to the target-site consensus sequence", PROC. NATL. ACAD. SCI, vol. 89, September 1992 (1992-09-01), pages 7996 - 8000
  • TIAN ET AL.: "A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery", PROC. NATL. ACAD. SCI., vol. 97, no. 9, 25 April 2000 (2000-04-25), pages 4730 - 4735
  • KAST AND HILVERT: "Genetic selection strategies for generating and chracterizing catalysts.", PURE AND APPL. CHEM., vol. 68, no. 11, 1996, pages 2017 - 2024
  • HOTZ AND SCHWER: "Mutational Analysis of teh Yeast DEAH-Box Splicing Factor Prp16", GENETICS, vol. 149, June 1998 (1998-06-01), pages 807 - 815
  • KOZMINSKI ET AL.: "Functions and Functional Domainsof the GTPase Cdc42p", MOL. BIOL. CELL., vol. 11, January 2000 (2000-01-01), pages 339 - 354
  • MÖSSNER ET AL.: "Importance of Redox Potential for the in Vivo Function of the Cytoplasmic DIsulfide Reductant Thioredoxin from Escherichia coli", J. BIOL. CHEM., vol. 274, no. 36, 3 September 1999 (1999-09-03), pages 25254 - 25259
  • GRAUSCHOPF ET AL.: "Why is DsbA Such an Oxidizing DIsulfide Bond Catalyst?", CELL, vol. 83, 15 December 1995 (1995-12-15), pages 947 - 955
  • ZHANG ET AL: "Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening", PROC NATL ACAD SCI, vol. 94, April 1997 (1997-04-01), pages 4504 - 4509
  • CHRISTIANS ET AL: "Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling", NATURE BIOTECHNOLOGY, vol. 17, March 1999 (1999-03-01), pages 259 - 264
  • VAGNER ET AL: "A vector for systematic gene inactivation in Bacillus subtilis.", MICROBIOLOGY, vol. 144, 1998, pages 3097 - 3104

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

DOCDB simple family (publication)

WO 0196551 A2 20011220; WO 0196551 A3 20020523; AU 6697801 A 20011224; CA 2413022 A1 20011220; EP 1294869 A2 20030326

DOCDB simple family (application)

US 0119367 W 20010614; AU 6697801 A 20010614; CA 2413022 A 20010614; EP 01944583 A 20010614