EP 1295120 A2 20030326 - AGONIST VERSUS ANTAGONIST BINDING TO G PROTEIN-COUPLED RECEPTORS
Title (en)
AGONIST VERSUS ANTAGONIST BINDING TO G PROTEIN-COUPLED RECEPTORS
Title (de)
AGONISTEN GEGEN DIE AGONISTENBINDUNG VON G-PROTEIN GEKOPPELTEN REZEPTOREN
Title (fr)
FIXATION D'AGONISTE/ANTAGONISTE A DES RECEPTEURS COUPLES A LA PROTEINE G
Publication
Application
Priority
- US 0119871 W 20010621
- US 21357500 P 20000622
Abstract (en)
[origin: WO0198747A2] A method of characterizing the biophysical properties of G protein-coupled receptors in response to binding by ligands. The clone human delta opioid receptor immobilized in a solid-supported lipid bilayer was investigated by a method featuring coupled plasmon-waveguide resonance (CPWR) spectroscopy. The invention offers a highly sensitive method that directly monitors mass density, conformation, and molecular orientation changes occurring in anisotropic thin films, and allows direct determination of binding constants. Although both agonist and antagonist binding to the receptor cause increases in molecular ordering within the proteolipid membrane, only agonist binding induces an increase in thickness and molecular packing density of the membrane (10). This provides a method of discriminating between agonist and antagonist binding.
IPC 1-7
IPC 8 full level
G01N 33/483 (2006.01); G01N 21/27 (2006.01); G01N 21/41 (2006.01); G01N 33/543 (2006.01); G01N 33/566 (2006.01)
CPC (source: EP)
G01N 33/54373 (2013.01); G01N 33/566 (2013.01); G01N 2333/726 (2013.01)
Citation (search report)
See references of WO 0198747A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0198747 A2 20011227; WO 0198747 A3 20020411; AU 7004601 A 20020102; CA 2413081 A1 20011227; EP 1295120 A2 20030326; JP 2004507716 A 20040311
DOCDB simple family (application)
US 0119871 W 20010621; AU 7004601 A 20010621; CA 2413081 A 20010621; EP 01948579 A 20010621; JP 2002504459 A 20010621