EP 1299327 A2 20030409 - PHOSPHORYLATED POLYPEPTIDES AND USES RELATED THERETO
Title (en)
PHOSPHORYLATED POLYPEPTIDES AND USES RELATED THERETO
Title (de)
PHOSPHORYLIERTE POLYPEPTIDE UND IHRE VERWENDUNG
Title (fr)
POLYPEPTIDES PHOSPHORYLES ET UTILISATIONS CORRESPONDANTES
Publication
Application
Priority
- US 0117935 W 20010531
- US 20824000 P 20000531
- US 25529900 P 20001213
Abstract (en)
[origin: WO0192469A2] Methods to generate modified polypeptides, modified antibodies, stably phosphorylated modified polypeptides, stably phosphorylated modified antibodies, polynucleotide sequences encoding the polypeptides, and uses thereof are provided. A computer-aided molecular modeling method is also provided to generate modified phosphorylatable polypeptides, particularly monoclonal antibodies (MAbs) for use in the diagnosis and treatment of cancers and other diseases. The corresponding MAbs contain heterologous recognition sites for polypeptide kinases and can be labeled by an identifiable label, such as radio-isotope <32>P. The phosphate group(s) attached to the phosphorylated polypeptide is unusually stable due to engineered intramolecular interactions between the phosphate group and its neighbouring groups. Polynucleotide sequences which encode a monoclonal antibody containing sequences encoding a putative phosphorylation site, and methods for analyzing the biochemical properties of a polypeptide by using molecular modeling tools, are also disclosed.
IPC 1-7
C07C 1/00; G06F 17/50; G01N 33/68; G01N 33/534; C07K 1/13; C12N 15/10
IPC 8 full level
C12N 15/09 (2006.01); C07K 1/107 (2006.01); C07K 14/00 (2006.01); C07K 16/32 (2006.01); C07K 16/46 (2006.01); C07K 19/00 (2006.01); C12P 21/08 (2006.01); G06F 17/50 (2006.01)
CPC (source: EP)
C07K 1/1077 (2013.01); C07K 14/001 (2013.01); C07K 2299/00 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0192469 A2 20011206; WO 0192469 A3 20020516; AU 2001265343 B2 20060803; AU 6534301 A 20011211; CA 2410754 A1 20011206; EP 1299327 A2 20030409; EP 1299327 A4 20050302; JP 2003534790 A 20031125
DOCDB simple family (application)
US 0117935 W 20010531; AU 2001265343 A 20010531; AU 6534301 A 20010531; CA 2410754 A 20010531; EP 01939871 A 20010531; JP 2002500663 A 20010531