EP 1303750 A1 20030423 - METHOD AND DEVICE FOR MULTICOLOUR 2-PHOTON FLUORESCENCE COINCIDENCE ANALYSIS
Title (en)
METHOD AND DEVICE FOR MULTICOLOUR 2-PHOTON FLUORESCENCE COINCIDENCE ANALYSIS
Title (de)
VERFAHREN UND VORRICHTUNG ZUR MEHRFARBEN-2-PHOTONEN-FLUORESZENZ-KOINZIDENZANALYSE
Title (fr)
PROCEDE ET DISPOSITIF D'ANALYSE DE COINCIDENCE PAR FLUORESCENCE A DEUX PHOTONS POLYCHROME
Publication
Application
Priority
- DE 10035190 A 20000720
- EP 0108328 W 20010718
Abstract (en)
[origin: WO0208732A1] The invention relates to a method for fluorescence measurement of sample analytes which are marked by different fluorescent markers having spectrally different fluorescence emissions. Said method consists of the following steps: the sample (11) is illuminated in a measuring volume by a laser (20) for exciting the fluorescence emission of the at least two fluorescent markers, the sample in the measuring volume being illuminated by one individual laser line at the most with such a high excitement intensity that the fluorescent markers are excited together by means of 2-photon absorption. The florescence emissions are detected by at least two detection devices (31, 32) which are designed to detect light in different spectral areas according to the spectral fluorescence properties of the fluorescent markers. A cross-correlation and/or a coincidence analysis of the detector signals from the detection devices is carried out. The invention also relates to a measuring device for carrying out the above-mentioned method.
IPC 1-7
IPC 8 full level
G01N 21/64 (2006.01); G01J 3/28 (2006.01)
CPC (source: EP US)
B82Y 10/00 (2013.01 - EP US); B82Y 20/00 (2013.01 - EP US); G01N 21/6408 (2013.01 - EP US); G01J 2003/2866 (2013.01 - EP US); G01N 2021/6441 (2013.01 - EP US); Y10T 436/13 (2015.01 - EP US)
Citation (search report)
See references of WO 0208732A1
Citation (examination)
- DE 19533092 A1 19970313 - BASF AG [DE]
- BERLAND K.M.; SO P.T.C.: "Dual-Color Fluorescence Correlation Spectroscopy Using Two-Photon Excitation", BIOPHYSICAL JOURNAL, vol. 78, no. 1, 12 February 2002 (2002-02-12), pages 441, XP001027202
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0208732 A1 20020131; AU 8969001 A 20020205; DE 10035190 A1 20020207; DE 10035190 B4 20040415; DE 10035190 C5 20090716; EP 1303750 A1 20030423; US 2004022684 A1 20040205; US 7507582 B2 20090324
DOCDB simple family (application)
EP 0108328 W 20010718; AU 8969001 A 20010718; DE 10035190 A 20000720; EP 01969428 A 20010718; US 33303403 A 20030723