EP 1303751 A1 20030423 - DYES AND METHODS OF DETECTION OF NUCLEIC ACID IN IMMATURE RED BLOOD CELLS
Title (en)
DYES AND METHODS OF DETECTION OF NUCLEIC ACID IN IMMATURE RED BLOOD CELLS
Title (de)
FARBSTOFFE UND VERFAHREN ZUR ERKENNUNG VON NUKLEINSÄURE IN UNREIFEN ROTEN BLUTKÖRPERCHEN
Title (fr)
COLORANTS ET PROCEDE DE DETECTION D'ACIDE NUCLEIQUE DANS DES HEMATIES IMMATURES
Publication
Application
Priority
- US 0114522 W 20010504
- US 56629800 A 20000505
Abstract (en)
[origin: WO0186264A1] The dyes of the present invention are useful for many purposes that include markers or tags for detecting the presence of a molecule or compound to which they are bound. The dyes may be either red-excitable or blue-excitable. The dyes of the invention are particularly well suited for staining of nucleic acids. For example, these dyes are particularly suitable for staining of RNA in reticulocytes. In another exemplary application, these dyes are suitable for staining DNA in nucleated red blood cells. Typically, when used in staining of nucleic acids, the dyes are formulated into reagent solutions. In addition, the invention provides compositions and methods for facilitating rapid transport of dye molecules through a cell membrane. Such rapid staining requires that a sample be contacted with a dye composition of the invention in the presence of at least one surfactant and optionally, a sulfonic acid or a salt thereof.
IPC 1-7
IPC 8 full level
G01N 33/48 (2006.01); C09B 23/00 (2006.01); C09B 23/02 (2006.01); C12Q 1/02 (2006.01); C12Q 1/68 (2006.01); G01N 1/30 (2006.01); G01N 21/47 (2006.01); G01N 21/64 (2006.01); G01N 33/483 (2006.01); G01N 33/58 (2006.01)
CPC (source: EP US)
C09B 23/02 (2013.01 - EP US); G01N 1/30 (2013.01 - EP US); G01N 21/47 (2013.01 - EP US); G01N 21/6428 (2013.01 - EP US); G01N 2001/302 (2013.01 - EP US); Y10S 436/80 (2013.01 - EP US); Y10T 436/10 (2015.01 - EP US); Y10T 436/101666 (2015.01 - EP US)
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
WO 0186264 A1 20011115; DE 60112585 D1 20050915; DE 60112585 T2 20060524; EP 1303751 A1 20030423; EP 1303751 A4 20030528; EP 1303751 B1 20050810; JP 2003532790 A 20031105; JP 4831914 B2 20111207; US 2002037589 A1 20020328; US 6368864 B1 20020409; US 6551831 B2 20030422
DOCDB simple family (application)
US 0114522 W 20010504; DE 60112585 T 20010504; EP 01933047 A 20010504; JP 2001583158 A 20010504; US 56629800 A 20000505; US 84915501 A 20010504