EP 1307589 A2 20030507 - METHOD FOR DETECTING MUTATIONS IN NUCLEOTIDE SEQUENCES
Title (en)
METHOD FOR DETECTING MUTATIONS IN NUCLEOTIDE SEQUENCES
Title (de)
VERFAHREN ZUM NACHWEIS VON MUTATIONEN IN NUCLEOTIDSEQUENZEN
Title (fr)
PROCEDE POUR DETECTER DES MUTATIONS DANS DES SEQUENCES NUCLEOTIDIQUES
Publication
Application
Priority
- DE 10038237 A 20000804
- EP 0108127 W 20010713
Abstract (en)
[origin: WO0212553A2] The invention relates to a method for simultaneously detecting mutations in different nucleotide sequences and for determining the transcription rate of mutated and non-mutated nucleotide sequences. The inventive method comprises the following steps: hybridizing single-stranded sample nucleotide sequences to single-stranded reference nucleotide sequences, fixating, before or during hybridization, single-stranded reference nucleotide sequences or single-stranded sample nucleotide sequences, or fixating, after or during hybridization, heteroduplices from reference and sample nucleotide sequences on an electronically addressable surface, incubating them with a substrate that recognizes heteroduplex mismatches, and detecting the substrate bonds.
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); B01J 19/00 (2006.01); C07H 21/00 (2006.01); C07K 14/195 (2006.01); C07K 14/245 (2006.01); C12N 9/00 (2006.01); C12N 15/09 (2006.01); C12N 15/52 (2006.01); C12Q 1/25 (2006.01); C12Q 1/34 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6883 (2018.01); G01N 33/566 (2006.01)
IPC 8 main group level
C12Q (2006.01)
CPC (source: EP US)
C12Q 1/6883 (2013.01 - EP US); C12Q 2600/156 (2013.01 - EP US)
Citation (search report)
See references of WO 0212553A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0212553 A2 20020214; WO 0212553 A3 20030130; AU 7063501 A 20020218; CA 2417861 A1 20030203; DE 10038237 A1 20020214; EP 1307589 A2 20030507; IL 154270 A0 20030917; JP 2004520010 A 20040708; US 2004110161 A1 20040610
DOCDB simple family (application)
EP 0108127 W 20010713; AU 7063501 A 20010713; CA 2417861 A 20010713; DE 10038237 A 20000804; EP 01949494 A 20010713; IL 15427001 A 20010713; JP 2002517837 A 20010713; US 34385903 A 20031124