Global Patent Index - EP 1311709 A4

EP 1311709 A4 20060802 - METHODS AND COMPOSITIONS FOR DIRECTED MOLECULAR EVOLUTION USING DNA-END MODIFICATION

Title (en)

METHODS AND COMPOSITIONS FOR DIRECTED MOLECULAR EVOLUTION USING DNA-END MODIFICATION

Title (de)

METHODEN UND ZUSAMMENSETZUNGEN ZUR GERICHTETEN MOLEKULAREN EVOLUTION WELCHE DNA-ENDMODIFIKATIONEN VERWENDEN

Title (fr)

METHODES ET COMPOSITIONS POUR L'EVOLUTION MOLECULAIRE DIRIGEE UTILISANT UNE MODIFICATION DES EXTREMITES DE L'ADN

Publication

EP 1311709 A4 20060802 (EN)

Application

EP 01965982 A 20010817

Priority

  • US 0125788 W 20010817
  • US 22647700 P 20000818

Abstract (en)

[origin: WO0216642A1] Methods as depicted in figure 3, for directed evolution are described where genetic elements are randomly cleaved to permit the deletion or addition of polynucleotides or both to create a library of related genetic elements with additions or deletions. Corresponding library populations are also described. These processes allow a significant sampling of sequence space which is necessary for directed evolution of genes. Further described are methods for effecting very small nucleotide deletions in genetic elements of interest.

IPC 1-7

C12Q 1/68; C12P 19/34; C12N 15/64; C07H 21/02

IPC 8 full level

C12N 15/09 (2006.01); C12N 15/10 (2006.01)

CPC (source: EP)

C12N 15/102 (2013.01); C12N 15/1027 (2013.01)

Citation (search report)

  • [YD] BIONDI RICHARD M ET AL: "Random insertion of GFP into the cAMP-dependent protein kinase regulatory subunit from Dictyostelium discoideum", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 21, 1 November 1998 (1998-11-01), pages 4946 - 4952, XP002187520, ISSN: 0305-1048
  • [YD] OSTERMEIER M ET AL: "A combinatorial approach to hybrid enzymes independent of DNA homology.", NATURE BIOTECHNOLOGY. DEC 1999, vol. 17, no. 12, December 1999 (1999-12-01), pages 1205 - 1209, XP002179317, ISSN: 1087-0156
  • [YD] CRAMERI A ET AL: "DNA SHUFFLING OF A FAMILY OF GENES FROM DIVERSE SPECIES ACCELERATESDIRECTED EVOLUTION", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 391, January 1998 (1998-01-01), pages 288 - 291, XP000919283, ISSN: 0028-0836
  • [T] KIM ET AL: "Directed Evolution and Identification of Control Regions of ColE1 Plasmid Replication Origins Using Only Nucleotide Deletions", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 351, no. 4, 26 August 2005 (2005-08-26), pages 763 - 775, XP005004656, ISSN: 0022-2836
  • See references of WO 0216642A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

DOCDB simple family (publication)

WO 0216642 A1 20020228; WO 0216642 A9 20030327; AU 8652801 A 20020304; CA 2419961 A1 20020228; EP 1311709 A1 20030521; EP 1311709 A4 20060802; JP 2004507247 A 20040311

DOCDB simple family (application)

US 0125788 W 20010817; AU 8652801 A 20010817; CA 2419961 A 20010817; EP 01965982 A 20010817; JP 2002522312 A 20010817