Global Patent Index - EP 1313756 A4

EP 1313756 A4 2004-12-22 - ANTIBODIES SPECIFIC FOR METHYLATED LYSINES IN HISTONES

Title (en)

ANTIBODIES SPECIFIC FOR METHYLATED LYSINES IN HISTONES

Title (de)

ANTIKÖRPER, DIE SPEZIFISCH FÜR METHYLIERTE LYSINE IN HISTONEN SIND

Title (fr)

ANTICORPS SPECIFIQUES AUX LYSINES METHYLEES DANS DES HISTONES

Publication

EP 1313756 A4 (EN)

Application

EP 01964349 A

Priority

  • US 0126283 W
  • US 22776700 P
  • US 30274701 P

Abstract (en)

[origin: WO0218418A1] The present invention relates to the generation of methyllysine-specific histone antibodies. In particular, the H3 lysine 4 methylation specific antibody (Methyl(K4)H3) binds to histone H3 methylated at lysine 4. Methylation of lysine 4 (K4) on histone H3 has been associated with transcriptionally active regions of chromatin. A second antibody, H3 lysine 9 methylation specific antibody (Methyl(K9)H3) specifically binds to histone H3 methylated at lysine 9. Methylation of lysine 9 (K9) on histone H3 has been associated with gene silencing. These antibodies are useful in identifying regions of heterochromatin and euchromatin and serving as diagnostic and screening tools.

IPC 1-7 (main, further and additional classification)

C07K 7/00; A61K 38/04; A61K 39/395; C07K 16/18; G01N 33/53

IPC 8 full level (invention and additional information)

C12Q 1/02 (2006.01); C07K 7/06 (2006.01); C07K 14/47 (2006.01); C07K 16/18 (2006.01); C07K 16/44 (2006.01); C12P 21/08 (2006.01); C12Q 1/68 (2006.01); G01N 33/68 (2006.01)

CPC (invention and additional information)

C07K 16/18 (2013.01); C07K 14/47 (2013.01); G01N 33/6875 (2013.01); A61K 2039/505 (2013.01)

Citation (search report)

  • [Y] US 5989829 A 19991123 - PESTRONK ALAN [US]
  • [E] WO 03004050 A1 20030116 - UNIV VIRGINIA [US], et al
  • [X] MULLER S ET AL: "IMMUNOGENICITY OF FREE HISTONES AND OF HISTONES COMPLEXED WITH RNA", MOLECULAR IMMUNOLOGY, vol. 28, no. 7, 1991, pages 763 - 772, XP002280388, ISSN: 0161-5890
  • [X] STEMMER C ET AL: "Mapping of linear histone regions exposed at the surface of the nucleosome in solution", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 273, no. 1, 17 October 1997 (1997-10-17), pages 52 - 60, XP004461390, ISSN: 0022-2836
  • [X] WHITE D A ET AL: "Preparation of Site-Specific Antibodies to Acetylated Histones", METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 19, no. 3, November 1999 (1999-11-01), pages 417 - 424, XP004466844, ISSN: 1046-2023
  • [X] TURNER B M ET AL: "HISTONE H4 ACETYLATION IN HUMAN CELLS FREQUENCY OF ACETYLATION AT DIFFERENT SITES DEFINED BY IMMUNOLABELING WITH SITE-SPECIFIC ANTIBODIES", FEBS LETTERS, vol. 253, no. 1-2, 1989, pages 141 - 145, XP002280389, ISSN: 0014-5793
  • [X] TAPLICK J ET AL: "Histone H4 acetylation during interleukin-2 stimulation of mouse T cells", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 436, no. 3, 9 October 1998 (1998-10-09), pages 349 - 352, XP004258451, ISSN: 0014-5793
  • [Y] REA STEPHEN ET AL: "Regulation of chromatin structure by site-specific histone H3 methyltransferases", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 406, no. 6796, August 2000 (2000-08-01), pages 593 - 599, XP002154907, ISSN: 0028-0836
  • [XP] NAKAYAMA JUN-ICHI ET AL: "Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 292, no. 5514, 2001, pages 110 - 113, XP002177964, ISSN: 0036-8075
  • [YP] BRIGGS SCOTT D ET AL: "Histone H3 lysine 4 methylation is mediated by set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae", GENES AND DEVELOPMENT, vol. 15, no. 24, 15 December 2001 (2001-12-15), pages 3286 - 3295, XP002280395, ISSN: 0890-9369
  • [YP] STALLCUP MICHAEL R: "Role of protein methylation in chromatin remodeling and transcriptional regulation", ONCOGENE, vol. 20, no. 24 Review Issue 3, 28 May 2001 (2001-05-28), pages 3014 - 3020, XP002280396, ISSN: 0950-9232
  • [A] SIMS ROBERT J III ET AL: "Histone lysine methylation: A signature for chromatin function.", TRENDS IN GENETICS, vol. 19, no. 11, November 2003 (2003-11-01), pages 629 - 639, XP002280390, ISSN: 0168-9525 (ISSN print)
  • See also references of WO 0218418A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

WO 0218418 A1 20020307; AU 8521401 A 20020313; CA 2420277 A1 20020307; EP 1313756 A1 20030528; EP 1313756 A4 20041222; JP 2004520270 A 20040708

INPADOC legal status


2007-05-16 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20061122

2006-08-09 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20050622

2004-12-22 [A4] DESPATCH OF SUPPLEMENTARY SEARCH REPORT

- Effective date: 20041110

2004-07-21 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7C 07K 7/00 A

2004-07-21 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7C 07K 16/18 B

2004-07-21 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 33/53 B

2004-07-21 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7A 61K 38/04 B

2004-07-21 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7A 61K 39/395 B

2003-09-10 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: ALLIS, C., DAVID

2003-09-10 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: STRAHL, BRIAN, D.

2003-05-28 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20030324

2003-05-28 [AK] DESIGNATED CONTRACTING STATES:

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

2003-05-28 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Countries: AL LT LV MK RO SI