EP 1315459 A1 20030604 - METHOD FOR TRANSDERMAL NUCLEIC ACID SAMPLING
Title (en)
METHOD FOR TRANSDERMAL NUCLEIC ACID SAMPLING
Title (de)
VERFAHREN ZUR TRANSDERMALEN NUKLEINSÄUREENTNAHME
Title (fr)
METHODE D'ECHANTILLONNAGE D'ACIDE NUCLEIQUE TRANSDERMIQUE
Publication
Application
Priority
- US 0126102 W 20010820
- US 22767500 P 20000824
Abstract (en)
[origin: WO0215800A1] A minimally invasive transdermal nucleic acid sampling method comprises piercing through the outermost layer of the skin (20) and into the underlying epidermis with a plurality of microprojections (4). Living skin cells in the underlying epidermis are disrupted, causing them to release their contents including their nucleic acids (i.e., DNA, RNA, fragments thereof or other polynucleic acid material found in teh cleic and/or mitochondria of cells). The nucleic acid is collected on the surfaces of the microprojections and/or in a separate nucleic acid collection reservoir. The collected nucleic acid is then analyzed using standard polymerase chain reaction (PCR) techniques. Optionally a suction device (10) applies a partial vacuum through openings (8) in the microprojetion member (6) to the microcuts in the skin (20) for enhance defflux of intraelular and extracellular (i.e., body) fluids containing the nucleic acid.
IPC 1-7
IPC 8 full level
G01N 33/48 (2006.01); A61B 10/00 (2006.01); A61B 17/20 (2006.01); C12M 1/26 (2006.01); C12Q 1/24 (2006.01); C12Q 1/68 (2006.01); G01N 1/04 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP KR)
A61B 10/00 (2013.01 - KR); A61B 10/0045 (2013.01 - EP); A61B 17/205 (2013.01 - EP); A61B 2010/008 (2013.01 - EP)
Citation (search report)
See references of WO 0215800A1
Citation (examination)
- US 6063082 A 20000516 - DEVORE LAURI [US], et al
- US 6551338 B1 20030422 - CHIU RAY CHU-JENG [CA], et al
- US 5250067 A 19931005 - GELFER ALA [US], et al
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0215800 A1 20020228; AU 2001283469 B2 20060706; AU 8346901 A 20020304; CA 2420343 A1 20020228; CN 1471378 A 20040128; EP 1315459 A1 20030604; JP 2004506914 A 20040304; KR 20030068127 A 20030819; MX PA03001694 A 20041101
DOCDB simple family (application)
US 0126102 W 20010820; AU 2001283469 A 20010820; AU 8346901 A 20010820; CA 2420343 A 20010820; CN 01817873 A 20010820; EP 01962273 A 20010820; JP 2002520715 A 20010820; KR 20037002654 A 20030224; MX PA03001694 A 20010820