Global Patent Index - EP 1315459 A1

EP 1315459 A1 20030604 - METHOD FOR TRANSDERMAL NUCLEIC ACID SAMPLING

Title (en)

METHOD FOR TRANSDERMAL NUCLEIC ACID SAMPLING

Title (de)

VERFAHREN ZUR TRANSDERMALEN NUKLEINSÄUREENTNAHME

Title (fr)

METHODE D'ECHANTILLONNAGE D'ACIDE NUCLEIQUE TRANSDERMIQUE

Publication

EP 1315459 A1 20030604 (EN)

Application

EP 01962273 A 20010820

Priority

  • US 0126102 W 20010820
  • US 22767500 P 20000824

Abstract (en)

[origin: WO0215800A1] A minimally invasive transdermal nucleic acid sampling method comprises piercing through the outermost layer of the skin (20) and into the underlying epidermis with a plurality of microprojections (4). Living skin cells in the underlying epidermis are disrupted, causing them to release their contents including their nucleic acids (i.e., DNA, RNA, fragments thereof or other polynucleic acid material found in teh cleic and/or mitochondria of cells). The nucleic acid is collected on the surfaces of the microprojections and/or in a separate nucleic acid collection reservoir. The collected nucleic acid is then analyzed using standard polymerase chain reaction (PCR) techniques. Optionally a suction device (10) applies a partial vacuum through openings (8) in the microprojetion member (6) to the microcuts in the skin (20) for enhance defflux of intraelular and extracellular (i.e., body) fluids containing the nucleic acid.

IPC 1-7

A61B 17/20; A61B 10/00

IPC 8 full level

G01N 33/48 (2006.01); A61B 10/00 (2006.01); A61B 17/20 (2006.01); C12M 1/26 (2006.01); C12Q 1/24 (2006.01); C12Q 1/68 (2006.01); G01N 1/04 (2006.01); G01N 33/50 (2006.01)

CPC (source: EP KR)

A61B 10/00 (2013.01 - KR); A61B 10/0045 (2013.01 - EP); A61B 17/205 (2013.01 - EP); A61B 2010/008 (2013.01 - EP)

Citation (search report)

See references of WO 0215800A1

Citation (examination)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

DOCDB simple family (publication)

WO 0215800 A1 20020228; AU 2001283469 B2 20060706; AU 8346901 A 20020304; CA 2420343 A1 20020228; CN 1471378 A 20040128; EP 1315459 A1 20030604; JP 2004506914 A 20040304; KR 20030068127 A 20030819; MX PA03001694 A 20041101

DOCDB simple family (application)

US 0126102 W 20010820; AU 2001283469 A 20010820; AU 8346901 A 20010820; CA 2420343 A 20010820; CN 01817873 A 20010820; EP 01962273 A 20010820; JP 2002520715 A 20010820; KR 20037002654 A 20030224; MX PA03001694 A 20010820