Global Patent Index - EP 1315822 A2

EP 1315822 A2 2003-06-04 - MODIFIED CONSTRUCT DOWNSTREAM OF THE INITIATION CODON FOR RECOMBINANT PROTEIN OVEREXPRESSION

Title (en)

MODIFIED CONSTRUCT DOWNSTREAM OF THE INITIATION CODON FOR RECOMBINANT PROTEIN OVEREXPRESSION

Title (de)

EXPRESSIONSVEKTOREN MIT MUTIERTEM TRYPTOPHANOPERON-FRAGMENT

Title (fr)

VECTEURS D'EXPRESSION COMPRENANT UN FRAGMENT MODIFIE DE L'OPERON TRYPTOPHANE

Publication

EP 1315822 A2 (FR)

Application

EP 01947565 A

Priority

  • FR 0101952 W
  • FR 0008002 A

Abstract (en)

[origin: WO0198453A2] The invention concerns a construct for expressing a gene coding for a recombinant protein of interest placed under the control of a tryptophan operon (Ptrp) in a prokaryotic cell, comprising directly downstream of the initiation codon a nucleic sequence SEQ ID N<o> 1 and downstream of said sequence a multiple cloning cassette designed to receive the gene coding for said recombinant protein of interest, at least nucleic acids of the nucleic sequence SEQ ID N<o> 1 being mutated or deleted so as to enable overexpression of said recombinant protein. The invention also concerns a vector containing such a construct, a prokaryotic host cell transformed by said vector, as well as a method for producing a recombinant protein of interest using the inventive construct.

IPC 1-7 (main, further and additional classification)

C12N 15/71; C12N 15/67

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); A61K 38/00 (2006.01); C12N 1/21 (2006.01); C12N 15/71 (2006.01); C12P 21/02 (2006.01)

CPC (invention and additional information)

C12N 15/71 (2013.01)

Citation (search report)

See references of WO 0198453A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

WO 0198453 A2 20011227; WO 0198453 A3 20020801; AU 6922401 A 20020102; BR 0111907 A 20031230; CA 2413612 A1 20011227; CN 1443242 A 20030917; EP 1315822 A2 20030604; FR 2810675 A1 20011228; FR 2810675 B1 20020927; JP 2004500875 A 20040115; MX PA02012880 A 20030514; US 2004260060 A1 20041223

INPADOC legal status


2007-07-04 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20070103

2006-10-11 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20060908

2003-06-04 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20030324

2003-06-04 [AK] DESIGNATED CONTRACTING STATES:

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR