EP 1315822 A2 20030604 - MODIFIED CONSTRUCT DOWNSTREAM OF THE INITIATION CODON FOR RECOMBINANT PROTEIN OVEREXPRESSION
Title (en)
MODIFIED CONSTRUCT DOWNSTREAM OF THE INITIATION CODON FOR RECOMBINANT PROTEIN OVEREXPRESSION
Title (de)
EXPRESSIONSVEKTOREN MIT MUTIERTEM TRYPTOPHANOPERON-FRAGMENT
Title (fr)
VECTEURS D'EXPRESSION COMPRENANT UN FRAGMENT MODIFIE DE L'OPERON TRYPTOPHANE
Publication
Application
Priority
- FR 0101952 W 20010621
- FR 0008002 A 20000622
Abstract (en)
[origin: WO0198453A2] The invention concerns a construct for expressing a gene coding for a recombinant protein of interest placed under the control of a tryptophan operon (Ptrp) in a prokaryotic cell, comprising directly downstream of the initiation codon a nucleic sequence SEQ ID N<o> 1 and downstream of said sequence a multiple cloning cassette designed to receive the gene coding for said recombinant protein of interest, at least nucleic acids of the nucleic sequence SEQ ID N<o> 1 being mutated or deleted so as to enable overexpression of said recombinant protein. The invention also concerns a vector containing such a construct, a prokaryotic host cell transformed by said vector, as well as a method for producing a recombinant protein of interest using the inventive construct.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); A61K 38/00 (2006.01); C12N 1/21 (2006.01); C12N 15/71 (2006.01); C12P 21/02 (2006.01)
CPC (source: EP US)
C12N 15/71 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0198453 A2 20011227; WO 0198453 A3 20020801; AU 6922401 A 20020102; BR 0111907 A 20031230; CA 2413612 A1 20011227; CN 1443242 A 20030917; EP 1315822 A2 20030604; FR 2810675 A1 20011228; FR 2810675 B1 20020927; JP 2004500875 A 20040115; MX PA02012880 A 20030514; US 2004260060 A1 20041223
DOCDB simple family (application)
FR 0101952 W 20010621; AU 6922401 A 20010621; BR 0111907 A 20010621; CA 2413612 A 20010621; CN 01812988 A 20010621; EP 01947565 A 20010621; FR 0008002 A 20000622; JP 2002504602 A 20010621; MX PA02012880 A 20010621; US 31197603 A 20031119