Global Patent Index - EP 1320594 A2

EP 1320594 A2 2003-06-25 - EXBB-EXBD-TONB GENE CLUSTER OF PSEUDOMONAS PUTIDA DOT-T1E CONFERRING TOLERANCE TO AROMATIC COMPOUNDS AND RESISTANCE TO ANTIBIOTICS

Title (en)

EXBB-EXBD-TONB GENE CLUSTER OF PSEUDOMONAS PUTIDA DOT-T1E CONFERRING TOLERANCE TO AROMATIC COMPOUNDS AND RESISTANCE TO ANTIBIOTICS

Title (de)

TOLERANZ GEGENÜBER AROMATISCHEN VERBINDUNGEN UND ANTIBIOTIKARESISTENZ VERLEIHENDE GENGRUPPE EXBB-EXBD-TONB AUS PSEUDOMONAS PUTIDA DOT-T1E

Title (fr)

PROCEDES DE PRODUCTION DE P-HYDROXYBENZOATE DANS DES BACTERIES

Publication

EP 1320594 A2 (EN)

Application

EP 01981383 A

Priority

  • US 0131180 W
  • US 23687900 P

Abstract (en)

[origin: WO0229034A2] This invention relates to the isolation of a novel tonB operon from Pseudomonas putida. These genes are useful to render the cells more sensitive to antibiotics, toluene, pHBA, aromatic compounds, parabenes, and aromatic amino acids after inactivation with specific mutant allels or more tolerant to these compounds after overexpression with appropriate expression vector. These findings are important in the field of medicine and biotechnology and biocatalysis. In addition a screen to identify pHBA tolerant genes is provided and strains with significant tolerance to pHBA were identified. These strains are important for pHBA production.

[origin: WO0229034A2] This invention relates to the isolation of a novel tonB operon from <i>Pseudomonas putida</i>. These genes are useful to render the cells more sensitive to antibiotics, toluene, pHBA, aromatic compounds, parabenes, and aromatic amino acids after inactivation with specific mutant allels or more tolerant to these compounds after overexpression with appropriate expression vector. These findings are important in the field of medicine and biotechnology and biocatalysis. In addition a screen to identify pHBA tolerant genes is provided and strains with significant tolerance to pHBA were identified. These strains are important for pHBA production.

IPC 1-7 (main, further and additional classification)

C12N 15/00

IPC 8 full level (invention and additional information)

C07K 14/21 (2006.01); C12N 15/31 (2006.01); C12N 15/52 (2006.01); C12P 7/42 (2006.01)

CPC (invention and additional information)

C07K 14/21 (2013.01); C12N 15/52 (2013.01); C12P 7/42 (2013.01)

Citation (search report)

See references of WO 0229034A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

EPO simple patent family

WO 0229034 A2 20020411; WO 0229034 A3 20030410; AP 200302768 A0 20030331; AU 1302802 A 20020415; EA 200300426 A1 20040429; EP 1320594 A2 20030625; US 7348421 B1 20080325

INPADOC legal status


2005-10-12 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20050401

2004-04-07 [RAP1] TRANSFER OF RIGHTS OF AN APPLICATION

- Owner name: NORTH CAROLINA STATE UNIVERSITY

2003-10-29 [RAP1] TRANSFER OF RIGHTS OF AN APPLICATION

- Owner name: NORTH CAROLINA STATE UNIVERSITY

2003-08-20 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: RAMOS, JUAN, L.

2003-08-20 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: BEN-BASSAT, ARIE

2003-08-20 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: GODOY, PATRICIA,E.I. DUPONT DE NEMOURS AND CO.

2003-08-20 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: RAMOS-GONZALES, M., ISABEL

2003-08-20 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: DUQUE, ESTRELLA,E.I. DUPONT DE NEMOURS AND CO.

2003-08-06 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: RAMOS, JUAN, L.

2003-08-06 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: BEN-BASSAT, ARIE

2003-08-06 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: GODOY, PATRICIA,E.I. DUPONT DE NEMOURS AND CO.

2003-08-06 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: RAMOS-GONZALES, M., ISABEL

2003-08-06 [RIN1] INVENTOR CHANGED BEFORE GRANT

- Inventor name: DUQUE, ESTRELLA,E.I. DUPONT DE NEMOURS AND CO.

2003-06-25 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20030417

2003-06-25 [AK] DESIGNATED CONTRACTING STATES:

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

2003-06-25 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Countries: AL LT LV MK RO SI