EP 1325108 A1 20030709 - METHOD AND DEVICE FOR CREATING MICRO-ARRAYS
Title (en)
METHOD AND DEVICE FOR CREATING MICRO-ARRAYS
Title (de)
VERFAHREN UND VORRICHTUNG ZUR ERZEUGUNG VON MIKROARRAYS
Title (fr)
PROCEDE ET DISPOSITIF DE CREATION DE MICRO-RESEAUX
Publication
Application
Priority
US 0027925 W 20001010
Abstract (en)
[origin: CA2425634A1] A method and device are provided for simultaneously creating a plurality of identical micro-arrays of biological samples. The invention utilizes a plurality of substrates, each of which having a top side, a bottom side, and a pattern of through-holes. Each through-hole has a wider upper cross-section, a narrower lower cross-section, and a step or plateau formed in the transition area. When the substrates are stacked, through-holes are in registry and for m tunnels extending through the stack of substrates. Reagents of interest are caused to flow through the tunnels and deposit on the step or plateau area. A barrier layer may be provided to prevent leak-through between neighboring holes. After the desired reagents have been deposited, the substrates are separated. In this manner a series of micro-arrays, each capable of containi ng hundreds or thousands of biological samples such as cDNA fragments, is forme d simultaneously.
IPC 1-7
C12M 1/34; C12M 3/00; G01N 33/543; B01J 19/00; B01L 3/00; C12M 3/04
IPC 8 full level
G01N 33/53 (2006.01); B01J 19/00 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 3/04 (2006.01); C12N 15/09 (2006.01); G01N 33/566 (2006.01); G01N 37/00 (2006.01); C40B 40/06 (2006.01); C40B 40/10 (2006.01); C40B 60/14 (2006.01)
CPC (source: EP KR)
B01J 19/0046 (2013.01 - EP); C12Q 1/68 (2013.01 - KR); B01J 2219/00286 (2013.01 - EP); B01J 2219/00317 (2013.01 - EP); B01J 2219/00319 (2013.01 - EP); B01J 2219/00416 (2013.01 - EP); B01J 2219/00529 (2013.01 - EP); B01J 2219/00585 (2013.01 - EP); B01J 2219/00596 (2013.01 - EP); B01J 2219/00605 (2013.01 - EP); B01J 2219/00608 (2013.01 - EP); B01J 2219/00659 (2013.01 - EP); B01J 2219/00668 (2013.01 - EP); B01J 2219/00691 (2013.01 - EP); B01J 2219/00722 (2013.01 - EP); B01J 2219/00725 (2013.01 - EP); C40B 40/06 (2013.01 - EP); C40B 40/10 (2013.01 - EP); C40B 60/14 (2013.01 - EP)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
AU 2000280042 B2 20070301; AU 8004200 A 20020422; CA 2425634 A1 20020418; CA 2425634 C 20080318; CN 1242047 C 20060215; CN 1454253 A 20031105; EP 1325108 A1 20030709; EP 1325108 A4 20050831; JP 2004511761 A 20040415; KR 100891217 B1 20090401; KR 20040007402 A 20040124
DOCDB simple family (application)
AU 2000280042 A 20001010; AU 8004200 A 20001010; CA 2425634 A 20001010; CN 00819958 A 20001010; EP 00970705 A 20001010; JP 2002534477 A 20001010; KR 20037005085 A 20030410