EP 1327005 B1 20120613 - METHOD FOR SELECTING NUCLEIC ACIDS THAT BOND WITH HIGH-AFFINITY TO A TARGET
Title (en)
METHOD FOR SELECTING NUCLEIC ACIDS THAT BOND WITH HIGH-AFFINITY TO A TARGET
Title (de)
VERFAHREN ZUR SELEKTION HOCHAFFIN AN EIN TARGET BINDENDER NUKLEINSÄUREN
Title (fr)
PROCEDE DE SELECTION D'ACIDES NUCLEIQUES SE LIANT A UNE CIBLE AVEC UNE AFFINITE ELEVEE
Publication
Application
Priority
- DE 0103817 W 20011002
- DE 10049074 A 20001002
Abstract (en)
[origin: WO0229093A2] The invention relates to a method for selecting nucleic acids that bond with high affinity to a target from a mixture of nucleic acids. The method comprises the following steps: a) loading the interior of a column with the target molecules and immobilising the target molecules in said column; b) feeding the mixture of nucleic acids into a first end of the column, to create a defined volumetric flow of medium through the column, running from the first end to the second end of said column; c) the nucleic acids bond with the target molecules with an affinity that decreases as the distance from the first end of the column increases and are immobilised; d) the volumetric flow of medium through the column is stopped after a defined period; e) the column is divided into column segments by a plurality of partitions, a routing co-ordinate being allocated to each segment; f) the immobilised nucleic acids from at least one segment are desorbed in a non-specific manner and are extracted using the allocation of the routing co-ordinate allocated to the segment.
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6811 (2018.01); C12Q 1/6834 (2018.01)
CPC (source: EP US)
C12Q 1/6806 (2013.01 - EP US); C12Q 1/6811 (2013.01 - EP US); C12Q 1/6834 (2013.01 - EP US); Y10T 436/143333 (2015.01 - EP US)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
Designated extension state (EPC)
AL LT LV MK RO SI
DOCDB simple family (publication)
WO 0229093 A2 20020411; WO 0229093 A3 20030501; AU 2048602 A 20020415; DE 10049074 A1 20020418; EP 1327005 A2 20030716; EP 1327005 B1 20120613; JP 2004510441 A 20040408; JP 4940456 B2 20120530; US 2004053280 A1 20040318; US 2009269752 A1 20091029; US 2012231964 A1 20120913
DOCDB simple family (application)
DE 0103817 W 20011002; AU 2048602 A 20011002; DE 10049074 A 20001002; EP 01986324 A 20011002; JP 2002532661 A 20011002; US 201113270325 A 20111011; US 26207608 A 20081030; US 39846903 A 20031029