EP 1334353 A1 20030813 - METHOD FOR SEPARATING AND DETECTING PROTEINS BY MEANS OF ELECTROPHORESIS
Title (en)
METHOD FOR SEPARATING AND DETECTING PROTEINS BY MEANS OF ELECTROPHORESIS
Title (de)
VERFAHREN ZUR TRENNUNG UND DETEKTION VON PROTEINEN MITTELS ELEKTROPHORESE
Title (fr)
PROCEDE DE SEPARATION ET DE DETECTION DE PROTEINES PAR ELECTROPHORESE
Publication
Application
Priority
- DE 10056838 A 20001116
- DE 10120803 A 20010427
- DE 10135497 A 20010720
- EP 0113195 W 20011114
Abstract (en)
[origin: WO0240983A1] The invention relates to a method for separating and detecting proteins or protein samples of cellular origin. Said proteins are contained in a separation buffer solution and the following steps are carried out: the protein samples are separated into individual fractions in a first separating step by means of free flow electrophoresis, isoelectric focusing (IEF) or isotachophoresis, and are linked with a marker; the protein fractions are separated into at least one capillary by means of capillary electrophoresis in a second separating step; and at least one marker linked with the protein fractions is detected in the at least one capillary.
IPC 1-7
IPC 8 full level
G01N 27/447 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP US)
G01N 27/44726 (2013.01 - EP US); G01N 27/44773 (2013.01 - EP US); G01N 33/6803 (2013.01 - EP US); G01N 2550/00 (2013.01 - EP US)
Citation (search report)
See references of WO 0240983A1
Citation (examination)
- WEBER G; BOCEK P: "RECENT DEVELOPMENTS IN PREPARATIVE FREE FLOW ISOELECTRIC FOCUSING", ELECTROPHORESIS, vol. 19, no. 10, July 1998 (1998-07-01), WEINHEIM, DE, pages 1649 - 1653, XP001057209
- OH-ISHI M; SATOH M; MAEDA T: "PREPARATIVE TWO-DIMENSIONAL GEL ELECTROPHORESIS WITH AGAROSE GELS IN THE FIRST DIMENSION FOR HIGH MOLECULAR MASS PROTEINS", ELECTROPHORESIS, vol. 21, no. 9, May 2000 (2000-05-01), WEINHEIM, DE, pages 1653 - 1669, XP001056980
- WEBER G; BOCEK P: "Interval isotachophoresis for purification and isolation of ionogenic species", ELECTROPHORESIS, vol. 19, no. 18, WEINHEIM, DE, pages 3090 - 3093, XP007900331, DOI: doi:10.1002/elps.1150191808
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0240983 A1 20020523; AU 1702902 A 20020527; CA 2428372 A1 20020523; EP 1334353 A1 20030813; JP 2004514136 A 20040513; NO 20032209 D0 20030515; NO 20032209 L 20030715; US 2004031683 A1 20040219
DOCDB simple family (application)
EP 0113195 W 20011114; AU 1702902 A 20011114; CA 2428372 A 20011114; EP 01996736 A 20011114; JP 2002542861 A 20011114; NO 20032209 A 20030515; US 41646003 A 20030512