Global Patent Index - EP 1343877 A2

EP 1343877 A2 20030917 - STABLE COMPOSITION COMPRISING A NUCLEASE AND A PHOSPHATASE

Title (en)

STABLE COMPOSITION COMPRISING A NUCLEASE AND A PHOSPHATASE

Title (de)

STABILE ZUSAMMENSETZUNG UMFASSEND EINE NUKLEASE UND EINE PHOSPHATASE

Title (fr)

COMPOSITION STABLE COMPRENANT UNE NUCLEASE ET UNE PHOSPHATASE

Publication

EP 1343877 A2 20030917 (EN)

Application

EP 01924218 A 20010320

Priority

  • US 0108859 W 20010320
  • US 19081300 P 20000321

Abstract (en)

[origin: WO0170943A2] A composition containing a nuclease, preferably Exonuclease I, and a phosphatase, preferably Shrimp Alkaline Phosphatase, wherein the enzymes are combined in a single composition yet each enzyme retains significant functional activity over time. Combining Exonuclease I and Shrimp Alkaline Phosphatase into one composition allows simplified processing of amplified DNA to degrade residual primers and nucleotide triphosphates thereby facilitating subsequent DNA analysis.

IPC 1-7

C12N 9/16; C12N 9/22; C12Q 1/68

IPC 8 full level

C12N 15/09 (2006.01); C12N 9/16 (2006.01); C12N 9/22 (2006.01); C12Q 1/42 (2006.01); C12Q 1/44 (2006.01)

CPC (source: EP)

C12N 9/16 (2013.01); C12N 9/22 (2013.01)

Citation (search report)

See references of WO 0170943A2

Citation (examination)

  • WO 9306243 A1 19930401 - US BIOCHEMICAL CORP [US]
  • WO 0111085 A2 20010215 - WHITEHEAD BIOMEDICAL INST [US], et al
  • "ExoSAP-IT Protocol", 2000, Retrieved from the Internet <URL:http://sequencingfacility.med.monash.edu.au/pdf/Exosap.pdf> [retrieved on 20080731]

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

DOCDB simple family (publication)

WO 0170943 A2 20010927; WO 0170943 A3 20030717; AU 5089001 A 20011003; CA 2403438 A1 20010927; CA 2403438 C 20081021; EP 1343877 A2 20030917; EP 2803724 A1 20141119; JP 2004501611 A 20040122; JP 3803295 B2 20060802; NO 20024537 D0 20020920; NO 20024537 L 20021120

DOCDB simple family (application)

US 0108859 W 20010320; AU 5089001 A 20010320; CA 2403438 A 20010320; EP 01924218 A 20010320; EP 14173528 A 20010320; JP 2001569326 A 20010320; NO 20024537 A 20020920