EP 1349649 A2 20031008 - SINGLE MOLECULE SEQUENCING METHOD
Title (en)
SINGLE MOLECULE SEQUENCING METHOD
Title (de)
EINZELMOLEKÜL-SEQUENZIERUNGSVERFAHREN
Title (fr)
PROCEDE DE SEQUENCAGE PAR MOLECULE INDIVIDUELLE
Publication
Application
Priority
- DE 10031840 A 20000630
- DE 10065626 A 20001229
- EP 0107460 W 20010629
Abstract (en)
[origin: WO0202225A2] The invention relates to a method for single molecule sequencing of nucleic acids and to a device for carrying out said method.
[origin: WO0202225A2] The invention relates to a method for single molecule sequencing of nucleic acids that comprises the following steps: a) providing a support particle with a nucleic acid molecule immobilized thereon, substantially all nucleotide components of at least one base type in at least one strand of the nucleic acid molecule carrying a fluorescent label, b) introducing the support particle into a microchannel sequencing device, c) retaining the support particle in the sequencing device, d) progressively cleaving individual nucleotide components from the immobilized nucleic acid molecule, e) guiding the cleaved off nucleotide components through a microchannel by means of a hydrodynamic flow, and f) identifying the base sequence of the nucleic acid molecule by the order of the cleaved off nucleotide components. The invention also relates to a device for carrying out the inventive method.
IPC 1-7
B01J 19/00; G01N 27/447; C12Q 1/68; B01L 3/00; B07C 5/00; G01N 15/14
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6869 (2013.01 - EP US)
C-Set (source: EP US)
Citation (examination)
GÖSCH ET AL.: "HYDRODYNAMIC FLOW PROFILING IN MICROCHANNEL STRUCTURES BY SINGLE MOLECULE FLUORESCENCE CORRELATION SPECTROSCOPY", ANALYTICAL CHEMISTRY, vol. 72, no. 14, 10 June 2000 (2000-06-10), COLUMBUS, US, pages 3260 - 3265, XP002180726
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0202225 A2 20020110; WO 0202225 A3 20030424; WO 0202225 A9 20030807; AU 6910901 A 20020114; EP 1349649 A2 20031008; US 2005153284 A1 20050714
DOCDB simple family (application)
EP 0107460 W 20010629; AU 6910901 A 20010629; EP 01947427 A 20010629; US 31167304 A 20041206