EP 1349961 A2 20031008 - METHODS FOR DETERMINING THE BIOLOGICAL EFFECTS OF COMPOUNDS ON GENE EXPRESSION
Title (en)
METHODS FOR DETERMINING THE BIOLOGICAL EFFECTS OF COMPOUNDS ON GENE EXPRESSION
Title (de)
VERFAHREN ZUR BESTIMMUNG DER BIOLOGISCHEN EFFEKTE VON VERBINDUNGEN AUF DIE GENEXPRESSION
Title (fr)
PROCEDES DE DETERMINATION DES EFFETS BIOLOGIQUES DE COMPOSES SUR L'EXPRESSION GENETIQUE
Publication
Application
Priority
- US 0146927 W 20011109
- US 24833900 P 20001113
Abstract (en)
[origin: WO0238734A2] Methods for determining one or more biological effects of a compound on gene expression are described. These methods involve obtaining a nuclear extract from cells exposed to a compound and then combining the nuclear extract with a nucleic acid, or library of nucleic acids, containing one or more regulatory elements under conditions that allow formation of cis/trans complexes between the regulatory elements and components (e.g., DNA binding proteins) of the nuclear extract. The complexes so formed are then compared with complexes formed using nuclear extracts obtained the same but untreaed cells and the compound. Differences between the comlexes formed as a result of exposure of teh cells to the compound can then be assessed. The methods of th einvention are preferably carried out in a high throughput format, and are useful, for example, to assess efficacy, toxicity, and mechanism of action of a compound. Accordingly, the invention will be useful in developing new drugs, and in better understanding and improving compounds already in use or under development.
IPC 1-7
IPC 8 full level
G01N 33/68 (2006.01)
CPC (source: EP)
G01N 33/6875 (2013.01); G01N 2333/4703 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0238734 A2 20020516; WO 0238734 A3 20020711; AU 3251002 A 20020521; CA 2431047 A1 20020516; EP 1349961 A2 20031008; EP 1349961 A4 20050126
DOCDB simple family (application)
US 0146927 W 20011109; AU 3251002 A 20011109; CA 2431047 A 20011109; EP 01992034 A 20011109