EP 1352241 A1 20031015 - ABSORBANCE MULTIPLEX TECHNOLOGY
Title (en)
ABSORBANCE MULTIPLEX TECHNOLOGY
Title (de)
MULTIPLEX-ABSORPTIONSTECHNOLOGIE
Title (fr)
TECHNIQUE MULTIPLEX PAR ABSORBANCE
Publication
Application
Priority
- US 0149745 W 20011221
- US 25828600 P 20001221
- US 94782301 A 20010906
Abstract (en)
[origin: WO0250537A1] A method of simultaneously detecting and quantitating multiple target molecules in a single reaction well of a reaction surface. Antibodies to different target molecules are bound to a reaction surface. A sample solution believed to contain the target molecules in added to the reaction surface and incubated. During incubation, the target molecules from complexes with the bound antibodies. After a washing step, an enzyme-bound antibody mixture is added to the reaction surface. The enzyme-bound antibodies have an affinity for the target molecule/bound antibody complexes. The sequential addition of specific enzyme substrates permits the detection of multiple target molecules without poisoning any of the enzymes. A kit for the simultaneous detection of multiple target molecules is also disclosed.
IPC 1-7
IPC 8 full level
G01N 33/543 (2006.01)
CPC (source: EP)
G01N 33/54306 (2013.01)
Citation (search report)
See references of WO 0250537A1
Designated contracting state (EPC)
AT BE CH CY DE FR GB LI
DOCDB simple family (publication)
WO 0250537 A1 20020627; AU 3272202 A 20020701; CA 2432398 A1 20020627; EP 1352241 A1 20031015
DOCDB simple family (application)
US 0149745 W 20011221; AU 3272202 A 20011221; CA 2432398 A 20011221; EP 01992259 A 20011221