EP 1354063 A2 20031022 - DETECTING SPECIFIC NUCLEOTIDE SEQUENCES
Title (en)
DETECTING SPECIFIC NUCLEOTIDE SEQUENCES
Title (de)
DETEKTION SPEZIFISCHER NUKLEOTIDSEQUENZEN
Title (fr)
DETECTION DE SEQUENCES NUCLEOTIDIQUES SPECIFIQUES
Publication
Application
Priority
- US 0132630 W 20011024
- US 24267200 P 20001024
Abstract (en)
[origin: WO0234947A2] Oligonucleotide primers, each labeled with a molecular energy transfer pair, including an energy donor and an energy acceptor, are used to detect the presence of one or more identifying nucleotide sequences. Two sequence-specific oligonucleotide primers, one specific for a first identifying sequence and the other for the second identifying sequence, together with a reverse primer, are employed in a polymerase chain reaction mixture which contains a nucleic acid template. Using only its complementary identifying sequence as a template, each sequence-specific primer directs amplification. Resultant PCR products, representing the first and the second identifying sequence, are detected via PCR amplification which uses two MET-labeled primers, each specific for the PCR product representing either the first or the second identifying sequence, together with another primer that is complementary to the PCR products representing both identifying sequences.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6827 (2018.01)
CPC (source: EP)
C12Q 1/6827 (2013.01)
Citation (search report)
See references of WO 0234947A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0234947 A2 20020502; WO 0234947 A3 20030814; AU 1341602 A 20020506; CA 2426812 A1 20020502; EP 1354063 A2 20031022; JP 2004530410 A 20041007
DOCDB simple family (application)
US 0132630 W 20011024; AU 1341602 A 20011024; CA 2426812 A 20011024; EP 01981799 A 20011024; JP 2002537915 A 20011024