EP 1355858 A2 20031029 - METHOD FOR ORTHOGONAL ANALYTE STACKING/INJECTION SYSTEMS IN ELECTROPHORESIS
Title (en)
METHOD FOR ORTHOGONAL ANALYTE STACKING/INJECTION SYSTEMS IN ELECTROPHORESIS
Title (de)
VERFAHREN FÜR ORTHOGONALE ANALYTEN-STACKING/INJEKTIONSSYSTEME IN DER ELEKTROPHORESE
Title (fr)
PROCEDE CONVENANT POUR DES SYSTEMES DE TASSEMENT/INJECTION ORTHOGONAUX D'ANALYTES DANS UNE ELECTROPHORESE
Publication
Application
Priority
- US 0143259 W 20011119
- US 24961100 P 20001117
Abstract (en)
[origin: WO0248673A2] In the present capillary electrokinetic chromatograpy method, analytes are injected by electroosmotic flow directly from a sample matrix into a separation buffer containing an electrokinetic vector with an opposite mobility. Analytes can now be injected at the velocity of electroosmotic flow, but are retained at the interface of the sample matrix-co-ion and separation buffer micelle zones as analyte/micelle complexes. Manipulation of the injecting force and opposing stacking force allow greatly increased length or volume of injection. Concentrations of the micelle, methanol, and borate in the separation buffer were provided to increase maximum injection length of neutral analytes. Reducing the analyte velocity in the separation buffer without substantially decreasing the velocity of the analyte during injection from the sample vial allowed greatly extended sample plug injection lengths. It is further enabled to inject sample solvent volumes equivalent to about twenty times the effective capillary volume. Equations and algorithms describing the injection process and maximum injection lengths for this mode of stacking in electrokinetic capillary chromatography are introduced. Use of the present method provides for maximum electrokinetic stacking injection for a wide variety of analytes and separation systems.
IPC 1-7
C02F 1/40; C02F 11/00; C25B 11/00; C25B 13/00; C25B 9/00; G01N 27/27; G01N 27/403; G01N 27/453
IPC 8 full level
G01N 27/447 (2006.01); G01N 30/16 (2006.01); G01N 30/02 (2006.01)
CPC (source: EP)
G01N 27/44743 (2013.01); G01N 30/16 (2013.01); G01N 30/02 (2013.01); G01N 2030/162 (2013.01)
Citation (search report)
See references of WO 0248673A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0248673 A2 20020620; WO 0248673 A3 20020815; AU 4148002 A 20020624; EP 1355858 A2 20031029
DOCDB simple family (application)
US 0143259 W 20011119; AU 4148002 A 20011119; EP 01988144 A 20011119