EP 1356105 A2 20031029 - METHOD FOR IDENTIFYING COMPOUNDS OR LEAD STRUCTURES AGAINST RNA TARGET MOTIFS AND RNA/PROTEIN INTERACTIONS

Title (en)

METHOD FOR IDENTIFYING COMPOUNDS OR LEAD STRUCTURES AGAINST RNA TARGET MOTIFS AND RNA/PROTEIN INTERACTIONS

Title (de)

VERFAHREN ZUR IDENTIFIZIERUNG VON VERBINDUNGEN ODER LEITSTRUKTUREN GEGEN RNA-TARGET-MOTIVE UND RNA/PROTEIN WECHSELWIRKUNGEN

Title (fr)

PROCEDE POUR IDENTIFIER DES LIAISONS OU DES STRUCTURES INITIALES CONTRE DES MOTIFS CIBLES ARN ET DES INTERACTIONS ARN/PROTEINE

Publication

EP 1356105 A2 20031029 (DE)

Application

EP 01985820 A 20011121

Priority

DE 10057853 A 20001122
DE 10144355 A 20010910
EP 0113568 W 20011121

Abstract (en)

[origin: WO0242491A2] Disclosed is a method for identifying compounds (lead structures) which specifically bind to (a) desired RNA target motif and can inhibit or eliminate the function thereof or (b) suppress a compound associated with a desired RNA target motif and can thereby inhibit or eliminate the function thereof. The inventive method is based on the attachment of a ligand (=a compound to be identified) to a RNA target motif which is coupled to a modified ribozyme so that the ribozyme is transformed into an active or inactive conformation resulting in the cleaving of a signal-giving ribozyme substrate. The identified compounds enabling modification of the cellular function of the RNA target motifs enable specific medicaments to be produced. The invention also relates to a polynucleotide comprising a hammerhead ribozyme and an aptamer for a target molecule.The base pairing model of the polynucleotide, when the target molecule binds to the aptamer, is different from the base pairing model of the polynucleotide when the target molecule does not bind to the aptamer.

IPC 1-7

C12Q 1/68; C12N 15/11; A61K 31/70; A61K 31/00

IPC 8 full level

A61K 31/35 (2006.01); A61K 31/4439 (2006.01); A61K 31/7056 (2006.01); C12N 15/113 (2010.01)

CPC (source: EP US)

A61K 31/35 (2013.01 - EP US); A61K 31/4439 (2013.01 - EP US); A61K 31/7056 (2013.01 - EP US); C12N 15/113 (2013.01 - EP US); C12N 2310/111 (2013.01 - EP US); C12N 2310/121 (2013.01 - EP US)

Citation (search report)

See references of WO 0242491A2

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

DOCDB simple family (publication)

WO 0242491 A2 20020530; WO 0242491 A3 20030828; AU 3573702 A 20020603; EP 1356105 A2 20031029; US 2005202423 A1 20050915

DOCDB simple family (application)

EP 0113568 W 20011121; AU 3573702 A 20011121; EP 01985820 A 20011121; US 43256005 A 20050314