EP 1358346 A2 20031105 - REAL TIME QUANTITATIVE PCR WITH INTERCALATING DYE FOR SINGLE AND MULTIPLEX TARGET DNA
Title (en)
REAL TIME QUANTITATIVE PCR WITH INTERCALATING DYE FOR SINGLE AND MULTIPLEX TARGET DNA
Title (de)
QUANTITATIVE 'ECHTZEIT'-PCR UNTER VERWENDUNG EINES INTERKALIERENDEN FARBSTOFFS FÜR EINZELNE UND MEHRFACHE DNA-MATRIZEN
Title (fr)
PCR QUANTITATIVE EN TEMPS REEL UTILISANT UN COLORANT INTERCALANT POUR LA DETECTION D'UN OU DE MULTIPLES ADN
Publication
Application
Priority
- US 0107101 W 20010307
- US 18753700 P 20000307
Abstract (en)
[origin: WO0166799A2] The PCR-based, dsDNA quantification method monitors the fluorescence of a target, whose melting characteristics is predetermined, during each amplification cycle at selected time-points. Fluorescence is measured immediately after the annealing phase (FE at TE), immediately below (FMS at TMS) and above (FME at TME) the melting of the target/amplicon. A change in slope from a baseline slope (SB = -(FMS - FE)/(TMS -TE)) to a melting phase slope (SM = -(FME - FMS)/(TME - TMS) indicates a specific amplification. The number of amplification cycles (CT) it takes for the quantity (SM - SB) to become greater than zero correlates with the starting concentration of the target (C). The concentration of the target in a sample is determined by comparing the value of CT for the sample with a standard curve. By selecting targets with distinguishable melting curve characteristics, multiple targets can be simultaneously detected.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6851 (2018.01)
CPC (source: EP)
C12Q 1/6851 (2013.01)
Citation (search report)
See references of WO 0166799A2
Citation (examination)
WO 0208414 A1 20020131 - NAT INST OF ADVANCED IND SCIEN [JP], et al
Designated contracting state (EPC)
AT BE CH CY DE DK FR GB IT LI NL
DOCDB simple family (publication)
WO 0166799 A2 20010913; WO 0166799 A3 20030904; AU 5080301 A 20010917; EP 1358346 A2 20031105; JP 2004533801 A 20041111
DOCDB simple family (application)
US 0107101 W 20010307; AU 5080301 A 20010307; EP 01924120 A 20010307; JP 2001565402 A 20010307