EP 1360193 A1 20031112 - METHOD FOR PURIFYING THE HELICOBACTER ADHESIN-LIKE PROTEIN A (ALPA)
Title (en)
METHOD FOR PURIFYING THE HELICOBACTER ADHESIN-LIKE PROTEIN A (ALPA)
Title (de)
VERFAHREN ZUR REINIGUNG DES ADHESIN-LIKE PROTEINS A (ALPA) AUS HELICOBACTER
Title (fr)
PROCEDE DE PURIFICATION DE LA PROTEINE ADHESINE-LIKE A (ALPA) D'HELICOBACTER
Publication
Application
Priority
- FR 0200355 W 20020130
- FR 0101499 A 20010205
Abstract (en)
[origin: FR2820424A1] The invention concerns a method for purifying the <i>Helicobacter</i> adhesin-like protein A (AlpA) which consists in: (i) contacting an AlpA preparation and 2.5 to 3.5 M of guanidine with a hydrophobic interaction chromatography material, so that the AlpA is adsorbed on the material; and (ii) eluting the AlpA with a solution containing 3.5 to 4.5 M of guanidine. The AlpA preparation to be purified can be in particular derived from an <i>E. coli</i> culture capable of expressing AlpA in a high-level recombinant form, rAlpA being in the form of inclusion bodies, the latter being recovered and solubilized in the presence of guanidine, and optionally ammonium sulphate-precipitated for the purpose of preliminary purification. The hydrophobic interaction chromatography can be followed up by an anion exchange chromatography in the presence of 8 M of urea.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07K 1/16 (2006.01); C07K 1/18 (2006.01); C07K 1/34 (2006.01); C07K 14/205 (2006.01); C07K 14/47 (2006.01); C12P 21/02 (2006.01); A61K 39/00 (2006.01)
CPC (source: EP US)
C07K 14/205 (2013.01 - EP US); A61K 39/00 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
FR 2820424 A1 20020809; FR 2820424 B1 20040102; CA 2436889 A1 20020822; EP 1360193 A1 20031112; JP 2004520403 A 20040708; US 2004058402 A1 20040325; WO 02064622 A1 20020822
DOCDB simple family (application)
FR 0101499 A 20010205; CA 2436889 A 20020130; EP 02701347 A 20020130; FR 0200355 W 20020130; JP 2002564951 A 20020130; US 47078003 A 20030730