EP 1362121 A2 20031119 - NANOPARTICLES HAVING OLIGONUCLEOTIDES ATTACHED THERETO AND USES THEREFOR
Title (en)
NANOPARTICLES HAVING OLIGONUCLEOTIDES ATTACHED THERETO AND USES THEREFOR
Title (de)
NANOPARTIKEL VERBUNDEN MIT OLIGONUKLEOTIDEN UND VERWENDUNGEN DAVON
Title (fr)
NANOPARTICULES COMPORTANT DES OLIGONUCLEOTIDES FIXES DESSUS ET UTILISATIONS ASSOCIEES
Publication
Application
Priority
- US 0125237 W 20010810
- US 22463100 P 20000811
- US 25439200 P 20001208
- US 25523500 P 20001211
- US 76050001 A 20010112
- US 82027901 A 20010328
Abstract (en)
[origin: WO0218643A2] The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comrising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.
IPC 1-7
IPC 8 full level
G01N 33/483 (2006.01); C07H 21/00 (2006.01); C12M 1/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 21/78 (2006.01); G01N 27/04 (2006.01); G01N 27/06 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 33/58 (2006.01); G01N 37/00 (2006.01)
CPC (source: EP)
B82Y 15/00 (2013.01); B82Y 30/00 (2013.01); C07H 21/00 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6825 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6837 (2013.01); B01J 2219/00274 (2013.01)
Citation (search report)
See references of WO 0218643A2
Citation (examination)
- WO 0153799 A1 20010726 - NANOGEN INC [US]
- US 5849486 A 19981215 - HELLER MICHAEL JAMES [US], et al
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
Designated extension state (EPC)
AL LT LV MK RO SI
DOCDB simple family (publication)
WO 0218643 A2 20020307; WO 0218643 A3 20030904; WO 0218643 A9 20031106; AU 8124801 A 20020313; CA 2418502 A1 20020307; EP 1362121 A2 20031119; JP 2004525607 A 20040826
DOCDB simple family (application)
US 0125237 W 20010810; AU 8124801 A 20010810; CA 2418502 A 20010810; EP 01959723 A 20010810; JP 2002522548 A 20010810