EP 1363961 B1 20090429 - END GROUP ACTIVATED POLYMERS WITH OLIGONUCLEOTIDE LIGANDS
Title (en)
END GROUP ACTIVATED POLYMERS WITH OLIGONUCLEOTIDE LIGANDS
Title (de)
ENDGRUPPENAKTIVIERTE POLYMERE MIT OLIGONUCLEOTID-LIGANDEN
Title (fr)
POLYMERES ACTIVES A GROUPES TERMINAUX AYANT DES LIGANDS OLIGONUCLEOTIDIQUES
Publication
Application
Priority
- US 0203341 W 20020204
- US 26608101 P 20010202
Abstract (en)
[origin: WO02077159A2] A method for coimmobilizing two or more biomolecules on a substrate in a defined ratio is disclosed. The method uses a copolymer conjugated to a number, N, of different types of oligonucleotides. The copolymer can be adsorbed to the surface of the substrate. N types of oligonucleotides complementary to the copolymer-bound oligonucleotides can be conjugated to N types of biomolecules. The types of the copolymer-bound oligonucleotides can be mixed in a defined ratio then adsorbed to the surface. The biomolecule-bound complementary oligonucletides can be conjugated to the copolymer-oligonucleotides to create a substrate with the biomolecules coimmobilized in a defined ratio. The invention also relates to a substrate prepared by the method of the invention.
IPC 8 full level
C12N 11/14 (2006.01); C12N 15/09 (2006.01); A61L 27/00 (2006.01); C12M 1/00 (2006.01); C12N 5/00 (2006.01); C12N 11/06 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP US)
C12N 11/06 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 02077159 A2 20021003; WO 02077159 A3 20021114; AT E430194 T1 20090515; AU 2002305924 A1 20021008; CA 2435894 A1 20021003; CA 2435894 C 20140513; DE 60232135 D1 20090610; EP 1363961 A2 20031126; EP 1363961 A4 20041222; EP 1363961 B1 20090429; JP 2004524038 A 20040812; JP 4328533 B2 20090909; US 2004219541 A1 20041104; US 7645721 B2 20100112
DOCDB simple family (application)
US 0203341 W 20020204; AT 02733793 T 20020204; AU 2002305924 A 20020204; CA 2435894 A 20020204; DE 60232135 T 20020204; EP 02733793 A 20020204; JP 2002576602 A 20020204; US 47029603 A 20030728