EP 1364055 A2 20031126 - SOLUBILITY REPORTER GENE CONSTRUCTS
Title (en)
SOLUBILITY REPORTER GENE CONSTRUCTS
Title (de)
LÖSLICHKEITSREPORTERGEN-KONSTRUKTE
Title (fr)
CONSTRUCTIONS DE GENES RAPPORTEURS DE SOLUBILITE
Publication
Application
Priority
- US 0151426 W 20011121
- US 72134000 A 20001121
- US 32483301 P 20010924
Abstract (en)
[origin: WO02061041A2] The present invention provides polynucleotides that a protein solubility response promoter operatively liked to a reporter gene and a genetic reporter system comprising these polynucleotides together with an expression construct for a target protein. The invention also provides cells comprising polynucleotides of the invention and the genetic reporter system. These compositions are useful to monitor the solubility of a target protein in a cell and to identify mutations to the cell or mutations to a polynucleotide encoding the target protein that alters the solubility of the target protein. The invention further provides method to identify variations in a protein biosynthetic process that alter the solubility of a target protein and methods to screen an expression library of recombinant clones to identify clones that express soluble proteins. Finally, the invention discloses a novel method of identifying an antibiotic agent.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C07K 14/245 (2006.01); C12M 1/34 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/70 (2006.01); C12Q 1/02 (2006.01); C12Q 1/06 (2006.01); C12Q 1/68 (2006.01); G01N 33/15 (2006.01); G01N 33/53 (2006.01); G01N 37/00 (2006.01)
CPC (source: EP)
C07K 14/245 (2013.01); C12N 15/70 (2013.01); C12Q 1/6897 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 02061041 A2 20020808; WO 02061041 A3 20030904; WO 02061041 A9 20040513; CA 2428248 A1 20020808; EP 1364055 A2 20031126; EP 1364055 A4 20040825; JP 2004535772 A 20041202
DOCDB simple family (application)
US 0151426 W 20011121; CA 2428248 A 20011121; EP 01998134 A 20011121; JP 2002561598 A 20011121