EP 1372750 A1 20040102 - METHOD FOR PREPARING A CARTILAGINOUS NEO-TISSUE
Title (en)
METHOD FOR PREPARING A CARTILAGINOUS NEO-TISSUE
Title (de)
VERFAHREN ZUR HERSTELLUNG EINES NEUEN KNORPELGEWEBES
Title (fr)
PROCEDE DE PREPARATION D'UN NEO-TISSU CARTILAGINEUX
Publication
Application
Priority
- FR 0201121 W 20020329
- FR 0104405 A 20010330
Abstract (en)
[origin: WO02078760A1] The invention concerns the preparation of a cartilaginous neo-tissue capable of being grafted. The method consists in: a) culturing chondrogenic cells, which are either autologous chondrocytes or cells precursors of chondrocytes prepared in vitro from pluripotent stem cells; b) contacting said chondrogenic cells with a chitosan hydrogel, having preferably a degree of acetylation ranging between 40 and 60 %, whereof the amphiphilic properties and the degree of acetylation is such that said cells adhere naturally to the outer surface of said hydrogel; c) covering the hydrogel/cell assembly thus obtained with a culture medium; and d) allowing a cartilaginous neo-tissue to develop in contact with the chitosan hydrogel during a minimum period of two weeks, frequently renewing the culture medium.
IPC 1-7
IPC 8 full level
A61L 27/20 (2006.01); A61L 27/38 (2006.01); A61L 27/52 (2006.01); C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/077 (2010.01); A61K 35/12 (2015.01)
CPC (source: EP US)
A61L 27/20 (2013.01 - EP US); A61L 27/3633 (2013.01 - EP US); A61L 27/3654 (2013.01 - EP US); A61L 27/3817 (2013.01 - EP US); A61L 27/3852 (2013.01 - EP US); A61L 27/52 (2013.01 - EP US); C12N 5/0068 (2013.01 - EP US); C12N 5/0655 (2013.01 - EP US); A61K 35/12 (2013.01 - EP US); A61L 2430/06 (2013.01 - EP US); C12N 2533/72 (2013.01 - EP US)
Citation (search report)
See references of WO 02078760A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 02078760 A1 20021010; CA 2442732 A1 20021010; EP 1372750 A1 20040102; FR 2822842 A1 20021004; FR 2822842 B1 20041119; US 2004171151 A1 20040902
DOCDB simple family (application)
FR 0201121 W 20020329; CA 2442732 A 20020329; EP 02757749 A 20020329; FR 0104405 A 20010330; US 47337004 A 20040401