EP 1379700 A2 20040114 - MICROARRAY METHOD FOR ENRICHING DNA FRAGMENTS FROM COMPLEX MIXTURES
Title (en)
MICROARRAY METHOD FOR ENRICHING DNA FRAGMENTS FROM COMPLEX MIXTURES
Title (de)
MIKROARRAY-VERFAHREN ZUR ANREICHERUNG VON DNA-FRAGMENTEN AUS KOMPLEXEN MISCHUNGEN
Title (fr)
PROCEDE A MICRORESEAU PERMETTANT D'ENRICHIR DES FRAGMENTS D'ADN PROVENANT DE MELANGES COMPLEXES
Publication
Application
Priority
- DE 10119468 A 20010412
- EP 0204056 W 20020411
Abstract (en)
[origin: WO02083943A2] The invention relates to a method for the simultaneous, parallel, selective enrichment of various DNA sections that are obtained from various tissues by complex amplification and that have desired individual sequence properties. The properties of the DNA fragments, especially the presence of 5-methylcytosines, can be identified by hybridization on DNA microarrays. The desired DNA fragments are enriched by repeating several times the process steps (hybridization, dehybridization and reamplification). The inventive method combines the SELEX method with complex DNA arrays that are used for enriching the DNA fragments. The amplificates are finally analyzed for their sequences.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6811 (2018.01); C12Q 1/6837 (2018.01)
CPC (source: EP US)
C12Q 1/6811 (2013.01 - EP US); C12Q 1/6837 (2013.01 - EP US)
Citation (search report)
See references of WO 02083943A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 02083943 A2 20021024; WO 02083943 A3 20031030; DE 10119468 A1 20021024; EP 1379700 A2 20040114; US 2005009020 A1 20050113
DOCDB simple family (application)
EP 0204056 W 20020411; DE 10119468 A 20010412; EP 02737945 A 20020411; US 47477904 A 20040520