EP 1381680 A1 20040121 - TEMPLATE-MEDIATED, LIGATION-ORIENTED METHOD OF NONRANDOMLY SHUFFLING POLYNUCLEOTIDES
Title (en)
TEMPLATE-MEDIATED, LIGATION-ORIENTED METHOD OF NONRANDOMLY SHUFFLING POLYNUCLEOTIDES
Title (de)
MATRIZEN-VERMITTELTES, LIGATION-ORIENTIERTES VERFAHREN DES NICHT ZUFÄLLIGEN SHUFFLING VON POLYNUKLEOTIDEN
Title (fr)
PROCEDE MATRICIEL A LIGATURE ORIENTEE DESTINE AU REARRANGEMENT NON ALEATOIRE DE POLYNUCLEOTIDES
Publication
Application
Priority
- FR 0105573 A 20010425
- IB 0202778 W 20020425
- US 28599801 P 20010425
- US 84086101 A 20010425
Abstract (en)
[origin: WO02086121A1] Method of gene shuffling oriented ligation, whereby at least two fragments are adjacently hybridized on an assembly template. Invention is particularly aimed at generating novel polynucleotides that differ in some advantageous respect compared to a reference sequence. Invention further includes sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.
IPC 1-7
IPC 8 full level
C07K 14/00 (2006.01); C12M 1/00 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12N 15/1027 (2013.01)
Citation (search report)
See references of WO 02086121A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 02086121 A1 20021031; AU 2002338443 B2 20070719; CA 2445258 A1 20021031; EP 1381680 A1 20040121; JP 2004531258 A 20041014; JP 4533584 B2 20100901
DOCDB simple family (application)
IB 0202778 W 20020425; AU 2002338443 A 20020425; CA 2445258 A 20020425; EP 02743543 A 20020425; JP 2002583635 A 20020425