EP 1402076 B1 20120801 - METHODS FOR EVALUATING PATIENT TREATMENT BY INHIBITORS OF VIRUS ENTRY USING RECOMBINANT VIRUS ASSAYS
Title (en)
METHODS FOR EVALUATING PATIENT TREATMENT BY INHIBITORS OF VIRUS ENTRY USING RECOMBINANT VIRUS ASSAYS
Title (de)
VERFAHREN ZUR BEURTEILUNG EINER PATIENTENBEHANDLUNG MIT INHIBITOREN DES VIRUSEINTRITTS MITTELS REKOMBINANTER VIRUS-ASSAYS
Title (fr)
METHODES D'EVALUATION DU TRAITEMENT D'UN PATIENT AVEC INHIBITEURS DE L'ENTREE D'UN VIRUS A L'AIDE DE DOSAGES DE VIRUS RECOMBINANTS
Publication
Application
Priority
- US 0218078 W 20020604
- US 87447501 A 20010604
- US 29587101 P 20010604
Abstract (en)
[origin: WO02099383A2] The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable singal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.
IPC 8 full level
C12Q 1/70 (2006.01); G01N 33/50 (2006.01); C12N 5/02 (2006.01); C12N 15/09 (2006.01); C12Q 1/02 (2006.01); C12Q 1/68 (2006.01); G01N 33/15 (2006.01)
CPC (source: EP)
C12Q 1/703 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 02099383 A2 20021212; WO 02099383 A3 20030828; WO 02099383 A8 20031211; BR 0210157 A 20041221; CN 1547618 A 20041117; EP 1402076 A2 20040331; EP 1402076 A4 20060104; EP 1402076 B1 20120801; ES 2396342 T3 20130220; JP 2004536594 A 20041209
DOCDB simple family (application)
US 0218078 W 20020604; BR 0210157 A 20020604; CN 02815323 A 20020604; EP 02756136 A 20020604; ES 02756136 T 20020604; JP 2003502458 A 20020604